FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006144294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2788227885
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGGAGCATCTCGTAT1225007404.39349813044424TruSeq Adapter, Index 2 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAAGGAGCATCTCGTATGCC125108850.4487038189132808TruSeq Adapter, Index 2 (96% over 33bp)
GAAGAGCACACGTCTGAACTCCAGTCACCAAGGAGCATCTCGTATGCCGT35826540.12849215156601163TruSeq Adapter, Index 2 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)