FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006144306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876269915
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC23945799727.326967741440715TruSeq Adapter, Index 3 (100% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCT221119412.523416657526123TruSeq Adapter, Index 3 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGT36363920.4149853758245255TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC30832430.35185996314845525TruSeq Adapter, Index 3 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC27202030.3104298063228611TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCACGTATGC18984770.2166543627142557TruSeq Adapter, Index 3 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG18979310.21659205314609026TruSeq Adapter, Index 3 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCCCGTATGC17040480.19446610808269046TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGCATCTCGTATGCC13909090.15873065777911594TruSeq Adapter, Index 3 (97% over 37bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC12326210.14066681725573107TruSeq Adapter, Index 3 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)