FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006144320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413637541
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC212850975.145832979410348TruSeq Adapter, Index 5 (100% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCT5350450.12935117027977883TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC4607150.11138133131876442TruSeq Adapter, Index 5 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)