FastQCFastQC Report
Sat 18 Jun 2022
EGAF00006144322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2348834887
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT1180956055.02783765915684TruSeq Adapter, Index 20 (97% over 44bp)
GAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGT49430130.2104453159887011TruSeq Adapter, Index 20 (97% over 39bp)
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT36134830.15384150754910003Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCCCGTAT29268180.12460722616983166TruSeq Adapter, Index 20 (97% over 44bp)
AAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTC26006790.11072208669897876TruSeq Adapter, Index 20 (97% over 38bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC24910480.10605462366840263No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)