FastQCFastQC Report
Tue 28 Jun 2022
EGAF00006144342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006144342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944882905
Sequences flagged as poor quality0
Sequence length150
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT32689922834.596797790515645TruSeq Adapter, Index 18 (97% over 40bp)
GAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGT80748310.8545853626169689TruSeq Adapter, Index 18 (97% over 35bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT54391370.5756413806639882TruSeq Adapter, Index 18 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG36363740.3848491681622709TruSeq Adapter, Index 18 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCC35183880.3723623299121916TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCCCGTAT34525570.3653952232313908TruSeq Adapter, Index 18 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA19224390.2034579088929543TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGCACATCTCGTATG13937680.1475069548432565TruSeq Adapter, Index 20 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCAATCTCGTATG13699690.1449882300495213TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA12610130.1334570657726102TruSeq Adapter, Index 18 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACGTCCGCACATCTCGTATG10822370.1145366261018343Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)