FastQCFastQC Report
Mon 6 Jun 2022
EGAF00006153136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006153136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5256204
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGGTGGTTTAGTGGTAGAATTTTTGTTTGTTACGTGGAATTCTCGG66703612.690451131653186No Hit
GGATGGGAGATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA2270574.3197904799737605RNA PCR Primer, Index 7 (97% over 35bp)
GTTTATGGTTATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAA1917823.6486787803517515No Hit
GTATTGGTGGTTTAGTGGTAGAATTTTTGTTTGTTACGTGGAATTCTCGG1630123.1013255954297057No Hit
AGTATTTGGATGGGAGATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1250892.3798353336362132RNA PCR Primer, Index 1 (100% over 28bp)
GGTTAGTATTTGGATGGGAGATTGTTTGGAATTCTCGGGTGCCAAGGAAC1042201.9827997543474338RNA PCR Primer, Index 1 (100% over 24bp)
GTATTGGTGGTTTAGTGGTAGAATTTTTGTTTTTTATGTGGAATTCTCGG915221.7412185676202827No Hit
GGGTTTGGTTAGTATTTGGATGGGAGATTGTTTGGAATTCTCGGGTGCCA650591.2377563732305672No Hit
GTATGGGTGGTTTAGTGGTAGAATTTTTGTTTGTTATGTGGAATTCTCGG609441.1594679354149877No Hit
GGGAGATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG559391.064247125872588RNA PCR Primer, Index 9 (100% over 39bp)
GGAGATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA542421.0319614687709988RNA PCR Primer, Index 9 (100% over 40bp)
GTATTTGGATGGGAGATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG523180.9953571056222323RNA PCR Primer, Index 1 (100% over 29bp)
GGTTTGGTTAGTATTTGGATGGGAGATTGTTTGGAATTCTCGGGTGCCAA500760.9527027489800624No Hit
GTTAGTATTTGGATGGGAGATTGTTTGGAATTCTCGGGTGCCAAGGAACT481270.915622757412003RNA PCR Primer, Index 1 (100% over 25bp)
AGGATTGTGGTTTTATTTTGTTGGTTTTTGGAATTCTCGGGTGCCAAGGA426980.8123352898783989RNA PCR Primer, Index 1 (100% over 22bp)
GAAGTTAAGTAGGGTTGGGTTTGGTTAGTATTTGGATGGGAGATTGTTTG358750.6825267816850336No Hit
AAGTTAGAATTTTGTTTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT353930.6733566657610702RNA PCR Primer, Index 1 (100% over 30bp)
AAGTAGGGTTGGGTTTGGTTAGTATTTGGATGGGAGATTGTTTGGAATTC346660.6595253913280382No Hit
TAGTATTTGGATGGGAGATTGTTTGGAATTCTCGGGTGCCAAGGAACTCC338180.6433920753456297RNA PCR Primer, Index 1 (100% over 27bp)
GAATGTGTTTGATTTTGTTTGATTTTGGAAGTTAAGTAGGGTTGGGTTTG305470.581160852965372No Hit
AGATAGTAGGATGGTGGTTATGGAAGTTGGAATTCTCGGGTGCCAAGGAA296290.5636957774089438RNA PCR Primer, Index 1 (100% over 23bp)
GATTTTGTTTGATTTTGGAAGTTAAGTAGGGTTGGGTTTGGTTAGTATTT273030.5194433092779503No Hit
GTTGGGTTTGGTTAGTATTTGGATGGGAGATTGTTTGGAATTCTCGGGTG265490.5050983561520824No Hit
AGAAAAGGTGTTGGTTGATATAGATAGTAGGATGGTGGTTATGGAAGTTG257800.49046802597463873No Hit
AATAATATAGGATTTTTTTGAGGTTTTGTAATTGGAATTCTCGGGTGCCA231740.44088851954756697No Hit
ATTTGGATGGGAGATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC200270.38101641412700116RNA PCR Primer, Index 1 (100% over 31bp)
GGTTATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAATTCTCG190780.36296155933065005No Hit
GTTTGGTTAGTATTTGGATGGGAGATTGTTTGGAATTCTCGGGTGCCAAG187580.3568735155637034No Hit
AGGGTTGGGTTTGGTTAGTATTTGGATGGGAGATTGTTTGGAATTCTCGG186710.35521832866456476No Hit
TTGATTTTGTTTGATTTTGGAAGTTAAGTAGGGTTGGGTTTGGTTAGTAT173150.32942024320212837No Hit
AATATAGGATTTTTTTGAGGTTTTGTAATTGGAATTCTCGGGTGCCAAGG166100.31600752177807406Illumina Small RNA Adapter 2 (100% over 21bp)
AAAGTAGGTTTGAGTTGTTTGGATATTGTAGTTAGGAATAATGGAATTCT164230.3124498212017646No Hit
ATAGGATTTTTTTGAGGTTTTGTAATTGGAATTCTCGGGTGCCAAGGAAC163440.3109468353967997RNA PCR Primer, Index 1 (100% over 24bp)
GTAGGGTTGGGTTTGGTTAGTATTTGGATGGGAGATTGTTTGGAATTCTC159230.3029372528159105No Hit
GTGTTTGATTTTGTTTGATTTTGGAAGTTAAGTAGGGTTGGGTTTGGTTA159000.3024996746701612No Hit
GTTTGGTTAGTTTAGTTGGTAGAGTATGGAATTCTCGGGTGCCAAGGAAC157720.30006445716338254RNA PCR Primer, Index 1 (100% over 24bp)
AGTAGGGTTGGGTTTGGTTAGTATTTGGATGGGAGATTGTTTGGAATTCT140490.26728414650572924No Hit
GTAGAATTTTTGTTTGTTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG138250.2630225158688666RNA PCR Primer, Index 1 (100% over 29bp)
AAGAAGTTTGGTTTGTTGGTGTGGAGTTGGGTGTGGAATTCTCGGGTGCC135290.25739107538444095No Hit
AGATTTTAGAAAAGGTGTTGGTTGATATAGATAGTAGGATGGTGGTTATG134850.2565539693664858No Hit
GGTTGGGTTTGGTTAGTATTTGGATGGGAGATTGTTTGGAATTCTCGGGT130880.2490009900681176No Hit
GATATAGATAGTAGGATGGTGGTTATGGAAGTTGGAATTCTCGGGTGCCA122270.2326203473076768No Hit
TAAGTTAGAATTTTGTTTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG121540.2312315123233421RNA PCR Primer, Index 1 (100% over 29bp)
AAAAGGTGTTGGTTGATATAGATAGTAGGATGGTGGTTATGGAAGTTGGA121050.2302992806215284No Hit
AAGGTGAGTTTAGGGAGGATAGAAATTTTTTGTGGAATTCTCGGGTGCCA120810.2298426773390074No Hit
TGATTTTGTTTGATTTTGGAAGTTAAGTAGGGTTGGGTTTGGTTAGTATT118040.2245727144532442No Hit
GATGGGAGATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT117630.22379268384560413RNA PCR Primer, Index 9 (100% over 36bp)
GTTTGATTTTGTTTGATTTTGGAAGTTAAGTAGGGTTGGGTTTGGTTAGT116880.22236579858772604No Hit
AAAGGTGTTGGTTGATATAGATAGTAGGATGGTGGTTATGGAAGTTGGAA113860.21662020728267015No Hit
AAGTTAAGTAGGGTTGGGTTTGGTTAGTATTTGGATGGGAGATTGTTTGG112970.21492697010998812No Hit
GTATTGGTGGTTTAGTGGTAGAATTTTTGTTTGTTATGTGGAATTCTCGG110560.21034191214800643No Hit
TTAGTATTTGGATGGGAGATTGTTTGGAATTCTCGGGTGCCAAGGAACTC108600.2066129853407516RNA PCR Primer, Index 1 (100% over 26bp)
TTAAGTTAGAATTTTGTTTAGGTGGAATTCTCGGGTGCCAAGGAACTCCA103900.1976711710580487RNA PCR Primer, Index 1 (100% over 28bp)
ATAGATAGTAGGATGGTGGTTATGGAAGTTGGAATTCTCGGGTGCCAAGG101660.19340954042118608Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGGAGATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC96330.18326914252186557RNA PCR Primer, Index 9 (100% over 37bp)
TTTGAATGTGTTTGATTTTGTTTGATTTTGGAATTCTCGGGTGCCAAGGA95690.18205153376847624RNA PCR Primer, Index 1 (100% over 22bp)
AGTTAAGTAGGGTTGGGTTTGGTTAGTATTTGGATGGGAGATTGTTTGGA94110.17904556215854633No Hit
TTTGAGGTTTTGTAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG93740.17834163209799317RNA PCR Primer, Index 6 (100% over 34bp)
AGTAGGATGGTGGTTATGGAAGTTGGAATTCTCGGGTGCCAAGGAACTCC93580.17803722990964582RNA PCR Primer, Index 1 (100% over 27bp)
AGGTTTGAGTTGTTTGGATATTGTAGTTAGGAATAATGGAATTCTCGGGT93430.17775185285807021No Hit
AGTGGTAGAATTTTTGTTTGTTACGTGGAATTCTCGGGTGCCAAGGAACT92520.17602056541184474RNA PCR Primer, Index 1 (100% over 25bp)
GGTTGATATAGATAGTAGGATGGTGGTTATGGAAGTTGGAATTCTCGGGT91490.1740609763243588No Hit
TTATGGTTATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAATT91070.17326192057994705No Hit
GGAGGATAGAAATTTTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC90910.1729575183915997RNA PCR Primer, Index 1 (100% over 31bp)
GTATGGGTGGTTTAGTGGTAGAATTTTTGTCTGTTACGTGGAATTCTCGG90360.17191113586915577No Hit
AAATGGGTAAGAAGTTTGGTTTGTTGGTGTGGAGTTGGGTGTGGAATTCT87260.16601334346992622No Hit
TAAGTAGGGTTGGGTTTGGTTAGTATTTGGATGGGAGATTGTTTGGAATT84200.16019165161778348No Hit
GGAGATATTATGATTATGAAGGTGGAATTCTCGGGTGCCAAGGAACTCCA83820.15946869642045858RNA PCR Primer, Index 1 (100% over 28bp)
AAATGGATTTTTGGAGTAGGGAGATGGAATTCTCGGGTGCCAAGGAACTC83210.15830816307738435RNA PCR Primer, Index 1 (100% over 26bp)
AGGGAGGATAGAAATTTTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG81190.1544650854494993RNA PCR Primer, Index 1 (100% over 29bp)
GTATGGGTGGTTCAGTGGTAGAATTTTTGTTTGTTACGTGGAATTCTCGG81090.15427483408178222No Hit
GGATATTGTAGTTAGGAATAATGGAATTCTCGGGTGCCAAGGAACTCCAG80730.1535899291580007RNA PCR Primer, Index 1 (100% over 29bp)
GTTTGATTTTGGAAGTTAAGTAGGGTTGGGTTTGGTTAGTATTTGGATGG79070.15043175645389714No Hit
TTGATTTTGGAAGTTAAGTAGGGTTGGGTTTGGTTAGTATTTGGATGGGA78410.14917609742696442No Hit
AGGTGTTGGTTGATATAGATAGTAGGATGGTGGTTATGGAAGTTGGAATT76260.14508569302104712No Hit
AAGGTGTTGGTTGATATAGATAGTAGGATGGTGGTTATGGAAGTTGGAAT75650.14392515967797292No Hit
GTTGGTTGATATAGATAGTAGGATGGTGGTTATGGAAGTTGGAATTCTCG73870.14053868533260885No Hit
TGATATAGATAGTAGGATGGTGGTTATGGAAGTTGGAATTCTCGGGTGCC71300.13564922518227984No Hit
TTGGGTTTGGTTAGTATTTGGATGGGAGATTGTTTGGAATTCTCGGGTGC69680.1325671530252631No Hit
GTATGGGTGGTTTAGTGGTAGAATTTTTGTTTGTCACGTGGAATTCTCGG68690.130683664484864No Hit
AATGTTTTTAAGTTAGAATTTTGTTTAGGTGGAATTCTCGGGTGCCAAGG68260.12986558360368053Illumina Small RNA Adapter 2 (100% over 21bp)
GGTAGAATTTTTGTTTGTTACGTGGAATTCTCGGGTGCCAAGGAACTCCA67960.12929482950052926RNA PCR Primer, Index 1 (100% over 28bp)
TATGGTTATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAATTC67710.1288192010812366No Hit
AGAAGTTTGGTTTGTTGGTGTGGAGTTGGGTGTGGAATTCTCGGGTGCCA67660.12872407539737804No Hit
TATTTGGATGGGAGATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT67410.12824844697808532RNA PCR Primer, Index 1 (100% over 30bp)
AATGTGTTTGATTTTGTTTGATTTTGGAAGTTAAGTAGGGTTGGGTTTGG66580.12666936062603354No Hit
GTAGGATGGTGGTTATGGAAGTTGGAATTCTCGGGTGCCAAGGAACTCCA66260.12606055624933887RNA PCR Primer, Index 1 (100% over 28bp)
GTTTTTAAGTTAGAATTTTGTTTAGGTGGAATTCTCGGGTGCCAAGGAAC65510.12463367099146076RNA PCR Primer, Index 1 (100% over 24bp)
TATAGGATTTTTTTGAGGTTTTGTAATTGGAATTCTCGGGTGCCAAGGAA65490.12459562071791734RNA PCR Primer, Index 1 (100% over 23bp)
TTTGTTTGATTTTGGAAGTTAAGTAGGGTTGGGTTTGGTTAGTATTTGGA63920.12160867424475914No Hit
ATATAGGATTTTTTTGAGGTTTTGTAATTGGAATTCTCGGGTGCCAAGGA63690.12117109609900986RNA PCR Primer, Index 1 (100% over 22bp)
GTATGGGTGGTTTAGTGGTAGAATTTTTGTTTGCTACGTGGAATTCTCGG63540.12088571904743423No Hit
ATGTTTTTAAGTTAGAATTTTGTTTAGGTGGAATTCTCGGGTGCCAAGGA63070.11999153761916394RNA PCR Primer, Index 1 (100% over 22bp)
GTTAAGTAGGGTTGGGTTTGGTTAGTATTTGGATGGGAGATTGTTTGGAA62230.11839342613034044No Hit
GTGGTTTAGTGGTAGAATTTTTGTTTGTTACGTGGAATTCTCGGGTGCCA61650.11728996819758138No Hit
ATGATTGAATGTTTTTAAGTTAGAATTTTGTTTAGGTGGAATTCTCGGGT61290.11660506327379988No Hit
GGTGGTTTAGTGGTAGAATTTTTGTTTGTTACGTGGAATTCTCGGGTGCC61250.11652896272671305No Hit
ATATTAAATGGATTTTTGGAGTAGGGAGATGGAATTCTCGGGTGCCAAGG61210.11645286217962621Illumina Small RNA Adapter 2 (100% over 21bp)
AGTTGTTTGGATATTGTAGTTAGGAATAATGGAATTCTCGGGTGCCAAGG61130.11630066108545255Illumina Small RNA Adapter 2 (100% over 21bp)
ATATTAAAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA60330.11477865014371588RNA PCR Primer, Index 9 (100% over 40bp)
AGTTAGAATTTTGTTTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC59060.11236245777370894RNA PCR Primer, Index 1 (100% over 31bp)
GTTTATGGTTATATTACTTTGAATGTGTTTGATTTTGTTTGATTTTGGAA57290.10899500856511657No Hit
GCATGGGTGGTTTAGTGGTAGAATTTTTGTTTGTTACGTGGAATTCTCGG57160.10874768178708437No Hit
GATTTTGGAAGTTAAGTAGGGTTGGGTTTGGTTAGTATTTGGATGGGAGA56910.10827205336779167No Hit
ATATAGATAGTAGGATGGTGGTTATGGAAGTTGGAATTCTCGGGTGCCAA56750.10796765117944433No Hit
GAAAAGGTGTTGGTTGATATAGATAGTAGGATGGTGGTTATGGAAGTTGG56550.10758714844401017No Hit
TAGATTTTAGAAAAGGTGTTGGTTGATATAGATAGTAGGATGGTGGTTAT56440.10737787193952138No Hit
TTTGATTTTGTTTGATTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA55960.10646466537447938RNA PCR Primer, Index 1 (100% over 32bp)
AAATTTTAAATGGGTAAGAAGTTTGGTTTGTTGGTGTGGAGTTGGGTGTG55770.10610318777581691No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGC55180.10498070470628612RNA PCR Primer, Index 9 (100% over 50bp)
TAAAGTAGGTTTGAGTTGTTTGGATATTGTAGTTAGGAATAATGGAATTC55120.10486655388565588No Hit
TTTAAGTTAGAATTTTGTTTAGGTGGAATTCTCGGGTGCCAAGGAACTCC54950.10454312656053685RNA PCR Primer, Index 1 (100% over 27bp)
GTTGATATAGATAGTAGGATGGTGGTTATGGAAGTTGGAATTCTCGGGTG54120.10296404020848506No Hit
AGATATTATGATTATGAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT53990.10271671343045286RNA PCR Primer, Index 1 (100% over 30bp)
TATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAATTCTCGGGT53960.10265963802013772No Hit
GAGATATTATGATTATGAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG53720.10220303473761673RNA PCR Primer, Index 1 (100% over 29bp)
TAATATAGGATTTTTTTGAGGTTTTGTAATTGGAATTCTCGGGTGCCAAG53660.10208888391698648No Hit
TTGTTTGATTTTGGAAGTTAAGTAGGGTTGGGTTTGGTTAGTATTTGGAT53370.10153715495060694No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)