FastQCFastQC Report
Thu 16 Jun 2022
EGAF00006153139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006153139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9365393
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATGGTTATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAA110453811.79382434885541No Hit
GTATGGGTGGTTTAGTGGTAGAATTTTTGTTTGTTACGTGGAATTCTCGG7576268.089633825297026No Hit
GTATTGGTGGTTTAGTGGTAGAATTTTTGTTTGTTACGTGGAATTCTCGG1890102.0181747845498847No Hit
GAATGTGTTTGATTTTGTTTGATTTTGGAAGTTAAGTAGGGTTGGGTTTG1750921.8695638293021979No Hit
GGATGGGAGATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT1607581.7165109889141865RNA PCR Primer, Index 1 (97% over 35bp)
GTATTGGTGGTTTAGTGGTAGAATTTTTGTTTTTTATGTGGAATTCTCGG1135831.2127948074362709No Hit
GATTTTGTTTGATTTTGGAAGTTAAGTAGGGTTGGGTTTGGTTAGTATTT1004181.072224091396912No Hit
GGTTAGTATTTGGATGGGAGATTGTTTGGAATTCTCGGGTGCCAAGGAAC988341.0553107595164453RNA PCR Primer, Index 1 (100% over 24bp)
AGTATTTGGATGGGAGATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA962411.0276237206489893RNA PCR Primer, Index 1 (100% over 28bp)
GTGTTTGATTTTGTTTGATTTTGGAAGTTAAGTAGGGTTGGGTTTGGTTA865370.9240082076641098No Hit
GGGAGATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC700330.7477849568085397RNA PCR Primer, Index 3 (100% over 39bp)
GTTTGATTTTGTTTGATTTTGGAAGTTAAGTAGGGTTGGGTTTGGTTAGT684770.731170597966364No Hit
GGGTTTGGTTAGTATTTGGATGGGAGATTGTTTGGAATTCTCGGGTGCCA678120.724069988306951No Hit
GTATGGGTGGTTTAGTGGTAGAATTTTTGTTTGTTATGTGGAATTCTCGG660750.7055229823243936No Hit
AAGTAGGGTTGGGTTTGGTTAGTATTTGGATGGGAGATTGTTTGGAATTC620330.6623640887253743No Hit
GGTTATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAATTCTCG576740.6158203932285597No Hit
GAAGTTAAGTAGGGTTGGGTTTGGTTAGTATTTGGATGGGAGATTGTTTG549250.586467647433482No Hit
ATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAAGTTAAGTAGG506960.5413120410430187No Hit
AATAATATAGGATTTTTTTGAGGTTTTGTAATTGGAATTCTCGGGTGCCA493300.5267264278178183No Hit
TTGATTTTGTTTGATTTTGGAAGTTAAGTAGGGTTGGGTTTGGTTAGTAT454300.4850837546272751No Hit
GTTAGTATTTGGATGGGAGATTGTTTGGAATTCTCGGGTGCCAAGGAACT451080.48164556468692765RNA PCR Primer, Index 1 (100% over 25bp)
AATGTGTTTGATTTTGTTTGATTTTGGAAGTTAAGTAGGGTTGGGTTTGG440230.4700603594531484No Hit
GTATTTGGATGGGAGATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG434170.46358972869584864RNA PCR Primer, Index 1 (100% over 29bp)
GGTTTGGTTAGTATTTGGATGGGAGATTGTTTGGAATTCTCGGGTGCCAA433760.46315194674692245No Hit
AGGATTGTGGTTTTATTTTGTTGGTTTTTGGAATTCTCGGGTGCCAAGGA423770.45248501584503714RNA PCR Primer, Index 1 (100% over 22bp)
GTTTGGTTAGTTTAGTTGGTAGAGTATGGAATTCTCGGGTGCCAAGGAAC387640.41390681629697756RNA PCR Primer, Index 1 (100% over 24bp)
TGATTTTGTTTGATTTTGGAAGTTAAGTAGGGTTGGGTTTGGTTAGTATT372840.398103955701592No Hit
GGTTATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAAGTTAAG367290.39217788297832246No Hit
GGAGATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCA366930.3917934890719482RNA PCR Primer, Index 3 (100% over 40bp)
TTATGGTTATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAATT349060.37271260266387113No Hit
ATGTGTTTGATTTTGTTTGATTTTGGAAGTTAAGTAGGGTTGGGTTTGGT335790.3585434161705761No Hit
TTATGGTTATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAAGT331810.3542937279834386No Hit
AGATAGTAGGATGGTGGTTATGGAAGTTGGAATTCTCGGGTGCCAAGGAA329670.35200871976221393RNA PCR Primer, Index 1 (100% over 23bp)
GTAGGGTTGGGTTTGGTTAGTATTTGGATGGGAGATTGTTTGGAATTCTC322200.34403254620494833No Hit
TAGTATTTGGATGGGAGATTGTTTGGAATTCTCGGGTGCCAAGGAACTCC307530.32836849452019795RNA PCR Primer, Index 1 (100% over 27bp)
GTTGGGTTTGGTTAGTATTTGGATGGGAGATTGTTTGGAATTCTCGGGTG287770.3072695401036561No Hit
AGTAGGGTTGGGTTTGGTTAGTATTTGGATGGGAGATTGTTTGGAATTCT283240.30243258344844687No Hit
ATGGTTATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAAGTTA262880.2806929725212813No Hit
AATATAGGATTTTTTTGAGGTTTTGTAATTGGAATTCTCGGGTGCCAAGG262300.28007367122767834Illumina Small RNA Adapter 2 (100% over 21bp)
TTATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAAGTTAAGTA260620.27827983299793185No Hit
AGGGTTGGGTTTGGTTAGTATTTGGATGGGAGATTGTTTGGAATTCTCGG251070.268082716870504No Hit
AAGTTAGAATTTTGTTTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT246080.26275459022381653RNA PCR Primer, Index 1 (100% over 30bp)
TATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAAGTTAAGTAG245790.2624449395770151No Hit
AGAAAAGGTGTTGGTTGATATAGATAGTAGGATGGTGGTTATGGAAGTTG238690.25486383753463415No Hit
ATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAATTCTCGGGTG238260.2544047003686871No Hit
TATGGTTATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAATTC234920.250838379126215No Hit
AAATTATTTATTTTTGATTTGGGGAGGTAGTGATGAAAAATAATAATATA227370.24277678470086628No Hit
ATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAAGTTAAGTAGGGT215490.23009178579051623No Hit
GTTTATGGTTATATTACTTTGAATGTGTTTGATTTTGTTTGATTTTGGAA214080.22858624299055041No Hit
GTTTGATTTTGGAAGTTAAGTAGGGTTGGGTTTGGTTAGTATTTGGATGG211480.22581006477784757No Hit
AAGTTAAGTAGGGTTGGGTTTGGTTAGTATTTGGATGGGAGATTGTTTGG204700.2185706462077993No Hit
ATGGTTATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAATTCT202960.21671274232699045No Hit
GTCTATGGTTATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAA199300.21280473761218563No Hit
GTGTTTGATTTTGTTTGATTTTGGAATTCTCGGGTGCCAAGGAACTCCAG197520.21090412329733518RNA PCR Primer, Index 1 (100% over 29bp)
TTTGTTTGATTTTGGAAGTTAAGTAGGGTTGGGTTTGGTTAGTATTTGGA196080.20936654767183824No Hit
ATTTATTTTTGATTTGGGGAGGTAGTGATGAAAAATAATAATATAGGATT195830.20909960745907832No Hit
GTTATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAAGTTAAGT190910.20384622407196365No Hit
TATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAATTCTCGGGT190440.2033443764719751No Hit
ATAGGATTTTTTTGAGGTTTTGTAATTGGAATTCTCGGGTGCCAAGGAAC183180.19559243269342783RNA PCR Primer, Index 1 (100% over 24bp)
TAAGTAGGGTTGGGTTTGGTTAGTATTTGGATGGGAGATTGTTTGGAATT182200.19454602705940904No Hit
AGTTAAGTAGGGTTGGGTTTGGTTAGTATTTGGATGGGAGATTGTTTGGA179970.1921649203615908No Hit
AAAGTAGGTTTGAGTTGTTTGGATATTGTAGTTAGGAATAATGGAATTCT173430.18518176439579204No Hit
TTTGAATGTGTTTGATTTTGTTTGATTTTGGAATTCTCGGGTGCCAAGGA172820.18453043027665791RNA PCR Primer, Index 1 (100% over 22bp)
TATGGTTATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAAGTT166870.17817725321297248No Hit
TTTGATTTTGTTTGATTTTGGAAGTTAAGTAGGGTTGGGTTTGGTTAGTA160960.17186678658332866No Hit
GGTTGGGTTTGGTTAGTATTTGGATGGGAGATTGTTTGGAATTCTCGGGT158650.16940025901742725No Hit
GATGGGAGATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA157690.16837520860042926RNA PCR Primer, Index 3 (100% over 36bp)
GTTTGGTTAGTATTTGGATGGGAGATTGTTTGGAATTCTCGGGTGCCAAG153690.164104165196271No Hit
GTTATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAATTCTCGG149860.16001464113678945No Hit
ATTTTGTTTGATTTTGGAAGTTAAGTAGGGTTGGGTTTGGTTAGTATTTG149530.1596622800559464No Hit
ATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAATTCTCGGGTGCC147460.15745201509429452No Hit
AGATTTTAGAAAAGGTGTTGGTTGATATAGATAGTAGGATGGTGGTTATG147230.1572064300985554No Hit
GTTTATGGTCATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAA146940.1568967794517539No Hit
GAATGTGTTTGATTTTGTTTGATTTTGGAATTCTCGGGTGCCAAGGAACT143670.15340520146885456RNA PCR Primer, Index 1 (100% over 25bp)
AAAAGGTGTTGGTTGATATAGATAGTAGGATGGTGGTTATGGAAGTTGGA142360.1520064347539927No Hit
TTGATTTTGGAAGTTAAGTAGGGTTGGGTTTGGTTAGTATTTGGATGGGA140720.15025530695828782No Hit
TTTGATTTTGTTTGATTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA138530.14791691069451116RNA PCR Primer, Index 1 (100% over 32bp)
GTTTGATTTTGTTTGATTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC136180.1454076726945682RNA PCR Primer, Index 1 (100% over 31bp)
GTTTATGGTTATACTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAA134380.14348570316269696No Hit
GATTTTGGAAGTTAAGTAGGGTTGGGTTTGGTTAGTATTTGGATGGGAGA133660.14271691534994846No Hit
GTAGAATTTTTGTTTGTTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG132630.1416171216733777RNA PCR Primer, Index 1 (100% over 29bp)
GATATAGATAGTAGGATGGTGGTTATGGAAGTTGGAATTCTCGGGTGCCA129540.13831774064366548No Hit
TTGTTTGATTTTGGAAGTTAAGTAGGGTTGGGTTTGGTTAGTATTTGGAT129420.13818960934154073No Hit
AAAGGTGTTGGTTGATATAGATAGTAGGATGGTGGTTATGGAAGTTGGAA127900.13656661284796057No Hit
GTTTATGGTTATATTATTTTGAACGTGTTTGATTTTGTTTGATTTTGGAA127120.13573375938414972No Hit
TAAGTTAGAATTTTGTTTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG120150.12829146625240392RNA PCR Primer, Index 1 (100% over 29bp)
TTAGTATTTGGATGGGAGATTGTTTGGAATTCTCGGGTGCCAAGGAACTC118190.1261986549843664RNA PCR Primer, Index 1 (100% over 26bp)
GTATTGGTGGTTTAGTGGTAGAATTTTTGTTTGTTATGTGGAATTCTCGG118160.1261666221588352No Hit
GGAGATATTATGATTATGAAGGTGGAATTCTCGGGTGCCAAGGAACTCCA114990.12278182026103977RNA PCR Primer, Index 1 (100% over 28bp)
GTTAAGTAGGGTTGGGTTTGGTTAGTATTTGGATGGGAGATTGTTTGGAA114520.12227997266105117No Hit
TTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAAGTTAAGTAGGGTT110790.11829722468667359No Hit
ATTTGGATGGGAGATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC110090.11754979209094589RNA PCR Primer, Index 1 (100% over 31bp)
TTAAGTTAGAATTTTGTTTAGGTGGAATTCTCGGGTGCCAAGGAACTCCA109630.11705862209946769RNA PCR Primer, Index 1 (100% over 28bp)
TAATATAGGATTTTTTTGAGGTTTTGTAATTGGAATTCTCGGGTGCCAAG109610.1170372668824469No Hit
ATATTAAAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCA108420.11576663146970981RNA PCR Primer, Index 3 (100% over 40bp)
GTTTATGGCTATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAA106050.11323603825274604No Hit
TTTGAGGTTTTGTAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT105820.11299045325700693RNA PCR Primer, Index 3 (100% over 34bp)
AAGGTGAGTTTAGGGAGGATAGAAATTTTTTGTGGAATTCTCGGGTGCCA104930.11204014609958172No Hit
AAGAAGTTTGGTTTGTTGGTGTGGAGTTGGGTGTGGAATTCTCGGGTGCC104730.1118265939293738No Hit
ATATAGGATTTTTTTGAGGTTTTGTAATTGGAATTCTCGGGTGCCAAGGA102380.10931735592943084RNA PCR Primer, Index 1 (100% over 22bp)
AAATGGATTTTTGGAGTAGGGAGATGGAATTCTCGGGTGCCAAGGAACTC101920.10882618593795264RNA PCR Primer, Index 1 (100% over 26bp)
TTATATTATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAATTCTCGGG100810.10764097139329871No Hit
ATAGATAGTAGGATGGTGGTTATGGAAGTTGGAATTCTCGGGTGCCAAGG99650.10640236880609281Illumina Small RNA Adapter 2 (100% over 21bp)
GTATGGGTGGTTTAGTGGTAGAATTTTTGTCTGTTACGTGGAATTCTCGG97800.10442701123166963No Hit
ATGGGAGATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG96730.10328450712105727RNA PCR Primer, Index 3 (100% over 37bp)
ATTTTGAATGTGTTTGATTTTGTTTGATTTTGGAAGTTAAGTAGGGTTGG95230.10168286584449793No Hit
TAGATTTTAGAAAAGGTGTTGGTTGATATAGATAGTAGGATGGTGGTTAT94360.1007539139040935No Hit
TGATTTTGTTTGATTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT94130.10050832890835441RNA PCR Primer, Index 3 (100% over 34bp)
AGTAGGATGGTGGTTATGGAAGTTGGAATTCTCGGGTGCCAAGGAACTCC93930.10029477673814649RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)