FastQCFastQC Report
Mon 6 Jun 2022
EGAF00006153141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006153141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53077
Sequences flagged as poor quality0
Sequence length100
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGT1019919.215479397855944No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACT938417.67997437684873No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGT843415.89012189837406No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACT836415.758238031539085No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTT6851.2905778397422611No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTT6181.1643461386287846No Hit
GGGGTGGAGCAAGCGTGACAAGTCCACGTATGACCCGACCGACGATATCG4250.8007234772123518No Hit
GGGGAACACGATGATATTCACCACACCGCTGGCGTTCAGCGCGTAGGCTT3540.6669555551368767No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTA3190.6010136217193888No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCGATAACT3080.5802890140738927No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTA2740.5162311358969045No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCGATAACT2730.5143470806564049No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTCATATTTTGAAAATGTTGGT2530.47666597584641185No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAGCCCAATAACT2460.46347758916291426No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCATAACTT2450.4615935339224146No Hit
AGTAAGGTTAGTTAAATAAGTTATGGGTTCATATTTTGAAAATGTTGGTT2370.4465210919984174No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTCATATTTTGAAAATGTTGGT2350.4427529815174181No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAACTTAT2270.4276805395934209No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTTATATTTTGAAAATGTTGGT2270.4276805395934209No Hit
CCTATGGTTTCAACCATGTACCGGATGTGTTCTGCCATGCGCTCCTGAAA2260.4257964843529212No Hit
CCTGCCCCAGCACCTGTTTGAATCGCTATGCATCGATCATTTGCAACGCC2010.37869510334042994No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCCATATTTTGAAAATGTTGGT1900.3579704956949338No Hit
AGTAAGGTTAGTTAAATAAGTTGGGTTCATATTTTGAAAATGTTGGTTAT1790.3372458880494376No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGGACCCAATAACT1780.33536183280893794No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATGAACCCAATAACT1770.3334777775684383No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAGCCCAATAACT1760.33159372232793866No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTCGAAAATGTTGGT1740.3278256118469393No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATCTTGAAAATGTTGGT1720.32405750136594No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATAAACCCAATAACT1670.3146372251634418No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAATATGAACCCAATAACT1660.31275316992294216No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATGAACCCAATAACT1650.3108691146824425No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTCTGAAAATGTTGGT1640.3089850594419429No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTTATATTTTGAAAATGTTGGT1610.30333289372044386No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATAAACCCAATAACT1600.30144883847994425No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATACTTTGAAAATGTTGGT1590.2995647832394446No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATGAACCCAATAACT1580.2976807279989449No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATCTTGAAAATGTTGGT1570.29579667275844523No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAGACCCAATAACT1570.29579667275844523No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCATAACTT1560.2939126175179456No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCTATATTTTGAAAATGTTGGT1520.286376396555947No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTCGAAAATGTTGGT1490.2807242308344481No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTAT1480.2788401755939484No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTAT1390.26188367842945154No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATAAACCCAATAACT1350.2543474574674529No Hit
AGTAAGGTTAGTTAAATAAGTTATGGGTTTATATTTTGAAAATGTTGGTT1350.2543474574674529No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATACTTTGAAAATGTTGGT1340.2524634022269533No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAATATAAACCCAATAACT1230.23173879458145713No Hit
AGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTATA1070.2015939107334627No Hit
CCTGCTTTTCGGGAAAACGGGATCACCACGATGGAACAGGTTAACGCAGG1040.19594174501196376No Hit
CCTCGCTTCCGGCAATACTCGTAAACCATATCAACCAGCTCGCTGACGTT1020.19217363453096445No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAACTTAT1000.18840552404996513No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTCTGAAAATGTTGGT980.18463741356896582No Hit
AATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTT870.16391280592346966No Hit
AGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTATA860.16202875068297No Hit
AGTAAGGTTAGTTAAATAAGTTGGGTTTATATTTTGAAAATGTTGGTTAT860.16202875068297No Hit
AATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTT770.14507225351847317No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTAACTAACCTTACT680.1281157563539763No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAAACTTA670.12623170111347665No Hit
TAATACAAAAAAAATATAACCAACATTTTTTTTGTATTAAGATCGGAAGA630.11869548015147804No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTATATTTTGAAAATGTTGGTT610.11492736967047873No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAACCCAATAACTT590.11115925918947943No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)