FastQCFastQC Report
Mon 6 Jun 2022
EGAF00006153147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006153147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70572
Sequences flagged as poor quality0
Sequence length100
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACT2281232.32443461996259No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGT2206731.268775151618204No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACT54187.677265771127359No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGT52347.4165391373349205No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTT12671.7953295924729353No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTA10051.4240775378337016No Hit
CAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTATTT5860.8303576489259196No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTT5440.7708439607776455No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTAT5330.7552570424530975No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTA3310.4690245423113983No Hit
AATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTT2480.35141415858980896No Hit
AGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTATA2310.32732528481550754No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAGCCCAATAACT2270.3216573145156719No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCGATAACT2140.30323641104120613No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATACTTTGAAAATGTTGGT1940.27489655954202796No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTCATATTTTGAAAATGTTGGT1920.2720625743921102No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCCATATTTTGAAAATGTTGGT1800.2550586634926033No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATGAACCCAATAACT1770.25080768576772655No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTCATATTTTGAAAATGTTGGT1740.24655670804284985No Hit
GGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTATAT1510.21396587881879497No Hit
TAATACAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTT1470.20829790851895935No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGGACCCAATAACT1390.19696196791928808No Hit
TAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTA1360.19271099019441137No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATGAACCCAATAACT1320.18704301989457575No Hit
ACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTATT1310.18562602731961683No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTCGAAAATGTTGGT1270.1799580570197812No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTCTGAAAATGTTGGT1250.17712407186986343No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATCTTGAAAATGTTGGT1240.17570707929490448No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTAT1170.16578813127019212No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAATATGAACCCAATAACT1110.1572861758204387No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATGAACCCAATAACT1070.15161820552060307No Hit
TAATACAAAAAAAATATCACCAACATTTTCAAAATATAAACCCAATAACT860.12186136144646603No Hit
TAATACAAAAAAAATATAACCAACATTTCAAAATATAAACCCAATAACTT810.11477639857167149No Hit
AATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTT760.10769143569687695No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)