FastQCFastQC Report
Mon 6 Jun 2022
EGAF00006153150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006153150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106412
Sequences flagged as poor quality0
Sequence length100
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGT3122129.33973611998647No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACT3072628.87456301920836No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGT1189411.177310829605684No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACT106209.980077434875765No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTT10620.9980077434875766No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTA9230.8673833778145321No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTT6870.6456038792617375No Hit
CAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTATTT5550.521557719054242No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTAT5300.4980641281058527No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTCATATTTTGAAAATGTTGGT5110.4802089989850769No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTCATATTTTGAAAATGTTGGT5050.4745705371574634No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATACTTTGAAAATGTTGGT4770.4482577152952675No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATGAACCCAATAACT4340.4078487388640379No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGGACCCAATAACT3750.3524038642258392No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTA3720.3495846333120325No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAGCCCAATAACT3700.34770514603616137No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCCATATTTTGAAAATGTTGGT3610.3392474532947412No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATCTTGAAAATGTTGGT3300.3101154005187385No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCGATAACT3110.2922602713979627No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTCGAAAATGTTGGT3060.28756155320828475No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATGAACCCAATAACT2810.2640679622598955No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAATATGAACCCAATAACT2360.22177949855279477No Hit
TAATACAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTT2330.2189602676389881No Hit
AGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTATA2330.2189602676389881No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTCTGAAAATGTTGGT2310.21708078036311693No Hit
AATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTT2150.2020448821561478No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATGAACCCAATAACT2070.19452693305266322No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTAT1560.1466000075179491No Hit
GGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTATAT1300.12216667293162425No Hit
TAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTA1130.10619103108671954No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)