FastQCFastQC Report
Wed 1 Jun 2022
EGAF00006153151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006153151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75641
Sequences flagged as poor quality0
Sequence length100
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACT2353131.108790206369562No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGT2347131.029468145582424No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACT65498.658002934916249No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGT63208.355257069578666No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTT12311.627424280482807No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTA11231.4846445710659562No Hit
CAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTATTT6040.7985087452572018No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTT5570.7363731309739427No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTAT5500.7271188905487764No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTA4180.55261035681707No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCGATAACT2560.3384407926917941No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAGCCCAATAACT2500.33050858661308025No Hit
AATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTT2330.30803400272339077No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATACTTTGAAAATGTTGGT2250.29745772795177217No Hit
AGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTATA2250.29745772795177217No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTCATATTTTGAAAATGTTGGT2110.2789492471014397No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATGAACCCAATAACT2100.27762721275498736No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCCATATTTTGAAAATGTTGGT1800.23796618236141776No Hit
TAATACAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTT1800.23796618236141776No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGGACCCAATAACT1770.23400007932206077No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTCATATTTTGAAAATGTTGGT1740.23003397628270383No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATCTTGAAAATGTTGGT1620.21416956412527596No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTCTGAAAATGTTGGT1600.21152549543237134No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATGAACCCAATAACT1460.19301701458203885No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATGAACCCAATAACT1360.17979667111751563No Hit
TAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTA1270.16789836199944475No Hit
GGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTATAT1220.16128819026718313No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAATATGAACCCAATAACT1200.1586441215742785No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTCGAAAATGTTGGT1170.15467801853492152No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTAT1150.1520339498420169No Hit
ACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTATT1120.14806784680265994No Hit
TAATACAAAAAAAATATCACCAACATTTTCAAAATATAAACCCAATAACT940.12427122856651816No Hit
AATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTT810.10708478206263798No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)