FastQCFastQC Report
Thu 16 Jun 2022
EGAF00006153152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006153152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75641
Sequences flagged as poor quality0
Sequence length100
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGT2423032.03289221453973No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACT2377631.43268862125038No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGT66488.788884335215029No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACT63668.416070649515474No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTA8111.0721698549728322No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTT7020.9280681112095293No Hit
CAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTATTT4140.5473222194312608No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTT3960.523525601195119No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTAT3900.5155933951164051No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTA2780.3675255483137452No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTCATATTTTGAAAATGTTGGT2640.3490170674634127No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTCATATTTTGAAAATGTTGGT2470.32654248357372323No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATACTTTGAAAATGTTGGT2280.3014238309911291No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATGAACCCAATAACT2220.29349162491241526No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAGCCCAATAACT2150.28423738448724895No Hit
AGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTATA1850.24457635409367937No Hit
TAATACAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTT1840.24325431974722705No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCCATATTTTGAAAATGTTGGT1780.2353221136685131No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGGACCCAATAACT1750.23135601062915617No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCGATAACT1750.23135601062915617No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATCTTGAAAATGTTGGT1600.21152549543237134No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAATATGAACCCAATAACT1580.2088814267394667No Hit
AATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTT1520.20094922066075277No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTCGAAAATGTTGGT1490.19698311762139578No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATGAACCCAATAACT1470.19433904892849116No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTCTGAAAATGTTGGT1340.177152602424611No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATGAACCCAATAACT1210.15996615592073082No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTAT960.1269152972594228No Hit
ACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTATT890.11766105683425655No Hit
GGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTATAT830.10972885075554263No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)