FastQCFastQC Report
Thu 16 Jun 2022
EGAF00006153153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006153153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50060
Sequences flagged as poor quality0
Sequence length100
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACT1608032.121454254894125No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGT1522830.419496604075107No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACT17573.5097882540950858No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTT14632.922493008389932No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGT14372.8705553335996803No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTA10332.0635237714742307No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCGATAACT6321.262485017978426No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATACTTTGAAAATGTTGGT6091.2165401518178185No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAGCCCAATAACT5421.0827007590890931No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATGAACCCAATAACT5221.0427487015581303No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTCATATTTTGAAAATGTTGGT5081.014782261286456No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTCATATTTTGAAAATGTTGGT4970.9928086296444267No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCCATATTTTGAAAATGTTGGT4960.9908110267678785No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATGAACCCAATAACT4440.8869356771873751No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATCTTGAAAATGTTGGT4350.8689572512984418No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGGACCCAATAACT4150.8290051937674789No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTCTGAAAATGTTGGT3880.7750699161006792No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATGAACCCAATAACT3330.6652017578905314No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAATATGAACCCAATAACT3240.6472233320015981No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTCGAAAATGTTGGT3220.6432281262485018No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTAT2920.5833000399520575No Hit
AATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTT1730.3455852976428286No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTT1230.2457051538154215No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTA1090.2177387135437475No Hit
CAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTATTT1040.20775069916100677No Hit
AGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTATA920.18377946464242909No Hit
TAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTA760.1518178186176588No Hit
TAATACAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTT760.1518178186176588No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTCATACTTTGAAAATGTTGGTT650.12984418697562924No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGCTCATATTTTGAAAATGTTGGTT560.11186576108669595No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)