FastQCFastQC Report
Mon 6 Jun 2022
EGAF00006153160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006153160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51854
Sequences flagged as poor quality0
Sequence length100
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGT1653831.89339298800478No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACT1618131.204921510394566No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGT18043.4789987271955876No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACT17053.288078065337293No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTT9061.7472133297334824No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTA7561.4579395996451576No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTCATATTTTGAAAATGTTGGT6321.218806649438809No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTCATATTTTGAAAATGTTGGT6261.2072357002352758No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATGAACCCAATAACT6091.174451344158599No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATACTTTGAAAATGTTGGT5651.0895977166660238No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCGATAACT5291.0201720214448258No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAGCCCAATAACT4790.9237474447487175No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGGACCCAATAACT4710.9083195124773402No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATCTTGAAAATGTTGGT4410.8504647664596753No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCCATATTTTGAAAATGTTGGT4160.8022524781116211No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATGAACCCAATAACT4130.7964670035098546No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTCGAAAATGTTGGT3910.7540401897635669No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAATATGAACCCAATAACT3560.6865429860762912No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTCTGAAAATGTTGGT3560.6865429860762912No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATGAACCCAATAACT3520.6788290199406024No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTAT2230.430053612064643No Hit
AATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTT1310.25263239094380374No Hit
AGTAAGGTTAGTTAAATAAGTTATGGGTTCATATTTTGAAAATGTTGGTT1190.22949049253673778No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCATAACTT980.18899217032437227No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTT940.18127820418868362No Hit
TAATACAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTT880.1697072549851506No Hit
CAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTATTT770.1484938481120068No Hit
AGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTATA710.13692289890847378No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTA680.1311374243067073No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATACTTTGAAAATGTTGGT670.12920893277278514No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCTATATTTTGAAAATGTTGGT630.12149496663709645No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTTATATTTTGAAAATGTTGGT620.11956647510317431No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATGAACCCAATAACTTA600.11570949203532996No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTTATATTTTGAAAATGTTGGT570.10992401743356348No Hit
TAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTA570.10992401743356348No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)