FastQCFastQC Report
Thu 16 Jun 2022
EGAF00006153161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006153161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53668
Sequences flagged as poor quality0
Sequence length100
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACT1717932.00976373257807No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGT1587829.585600357755087No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACT31975.956994857270627No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGT30105.608556309160021No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTT10401.9378400536632632No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTA8781.6359841991503317No Hit
CAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTATTT5210.9707833345755386No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTAT4730.8813445628680033No Hit
AGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTATA3040.5664455541477231No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTT2520.46955355146455985No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCGATAACT2300.42856078109860624No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTTATATTTTGAAAATGTTGGT2090.3894313184765596No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTA2090.3894313184765596No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCGATAACT1900.3540284713423269No Hit
AATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTT1860.34657524036669896No Hit
GGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTATAT1840.342848624878885No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1770.3298054706715361TruSeq Adapter, Index 9 (100% over 50bp)
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATAAACCCAATAACT1520.28322277707386156No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTCATATTTTGAAAATGTTGGT1420.2645896996347917No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAGCCCAATAACT1340.2496832376835358No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATGAACCCAATAACT1300.2422300067079079No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATACTTTGAAAATGTTGGT1290.2403666989640009No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAATATGAACCCAATAACT1280.23850339122009392No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATACTTTGAAAATGTTGGT1270.23664008347618692No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTTATATTTTGAAAATGTTGGT1250.23291346798837298No Hit
TAATACAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTT1230.229186852500559No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATGAACCCAATAACT1210.225460237012745No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTCATATTTTGAAAATGTTGGT1140.21241708280539612No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTCTGAAAATGTTGGT1110.2068271595736752No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTAT1060.19751062085414028No Hit
TAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTA1000.18633077439069837No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAGCCCAATAACT970.18074085115897742No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGGACCCAATAACT970.18074085115897742No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATAAACCCAATAACT920.1714243124394425No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCCATATTTTGAAAATGTTGGT860.1602444659760006No Hit
ACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTATT830.15465454274427964No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCTATATTTTGAAAATGTTGGT820.15279123500037267No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAACCCAATAACTT810.15092792725646567No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATAAACCCAATAACT790.1472013117686517No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATCTTGAAAATGTTGGT780.14533800402474473No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTCGAAAATGTTGGT770.14347469628083775No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATGAACCCAATAACT670.1248416188417679No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTATATTTTGAAAATGTTGGTT660.12297831109786092No Hit
AGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTATA650.12111500335395395No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTCGAAAATGTTGGT640.11925169561004696No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAGACCCAATAACT610.113661772378326No Hit
AGTAAGGTTAGTTAAATAAGTTATGGGTTCATATTTTGAAAATGTTGGTT600.11179846463441902No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATCTTGAAAATGTTGGT590.10993515689051205No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAACTTAT570.10620854140269806No Hit
AAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTAT570.10620854140269806No Hit
TAATACAAAAAAAATATAACCAACATTTCAAAATATAAACCCAATAACTT570.10620854140269806No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATGAACCCAATAACTTA550.1024819259148841No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCAATAACTT540.10061861817097711No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)