FastQCFastQC Report
Wed 1 Jun 2022
EGAF00006153163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006153163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70307
Sequences flagged as poor quality0
Sequence length100
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACT2062429.334205697867922No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGT1998428.423912270470936No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACT47546.761773365383247No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGT46866.665054688722318No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTT13711.9500192015019842No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTA11471.6314165019130387No Hit
CAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTATTT6770.9629197661683759No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTAT6440.9159827613182187No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTT4160.5916907278080419No Hit
AGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTATA4100.5831567269261951No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTA3640.5177293868320366No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC2650.3769183722815651TruSeq Adapter, Index 8 (100% over 50bp)
AATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTT2460.34989403615571707No Hit
GGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTATAT2430.3456270357147937No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATACTTTGAAAATGTTGGT2320.329981367431408No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATGAACCCAATAACT2290.32571436699048456No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTCATATTTTGAAAATGTTGGT2270.322869700029869No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAGCCCAATAACT2200.31291336566771444No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCGATAACT2190.31149103218740665No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCGATAACT2180.31006869870709886No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTCATATTTTGAAAATGTTGGT2030.288733696502482No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATAAACCCAATAACT2000.28446669606155855No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATACTTTGAAAATGTTGGT1960.27877736214032744No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTTATATTTTGAAAATGTTGGT1870.26597636081755727No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTAT1780.25317535949478714No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTTATATTTTGAAAATGTTGGT1640.23326269077047806No Hit
TAATACAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTT1620.23041802380986245No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAGCCCAATAACT1520.21619468900678454No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATGAACCCAATAACT1470.20908302160524556No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTCTGAAAATGTTGGT1450.20623835464462997No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATCTTGAAAATGTTGGT1450.20623835464462997No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTCGAAAATGTTGGT1390.19770435376278322No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATAAACCCAATAACT1380.19628202028247546No Hit
ACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTATT1360.19343735332185985No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTCGAAAATGTTGGT1350.19201501984155206No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCCATATTTTGAAAATGTTGGT1330.18917035288093645No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGGACCCAATAACT1330.18917035288093645No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATTTTGAAAATGTTGGTTA1240.17636935155816633No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCTATATTTTGAAAATGTTGGT1210.17210235111724292No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAACTTAT1190.16925768415662737No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAATATGAACCCAATAACT1190.16925768415662737No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATGAACCCAATAACT1170.1664130171960118No Hit
AGTAAGGTTAGTTAAATAAGTTGGGTTCATATTTTGAAAATGTTGGTTAT1140.16214601675508838No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAGACCCAATAACT1140.16214601675508838No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATGAACCCAATAACTTA1130.1607236832747806No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTATATTTTGAAAATGTTGGTT1110.157879016314165No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAATATAAACCCAATAACT1080.15361201587324166No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATAAACCCAATAACT1060.15076734891262605No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTCTGAAAATGTTGGT1050.14934501543231826No Hit
AGTAAGGTTAGTTAAATAAGTTATGGGTTTATATTTTGAAAATGTTGGTT1040.14792268195201047No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATCTTGAAAATGTTGGT1020.1450780149913949No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCATAACTT1020.1450780149913949No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCATAACTT990.1408110145504715No Hit
TAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTA990.1408110145504715No Hit
CAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTATTT980.13938868107016372No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAACCCAATAACTT950.13512168062924035No Hit
AGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTATA890.12658767974739357No Hit
AATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTT840.11947601234585462No Hit
AGTAAGGTTAGTTAAATAAGTTATGGGTTCATATTTTGAAAATGTTGGTT830.11805367886554682No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)