FastQCFastQC Report
Thu 16 Jun 2022
EGAF00006153167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006153167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71790
Sequences flagged as poor quality0
Sequence length100
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACT2154230.006964758322884No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGT2094229.171193759576543No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACT45056.275247248920462No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGT44516.200027859033291No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTT13921.938988717091517No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTA11091.544783396016158No Hit
CAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTATTT6740.9388494219250593No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTAT6130.8538793703858476No Hit
AGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTATA4300.5989692157682128No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTT3870.5390722941913916No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTA3410.47499651762083855No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTCATATTTTGAAAATGTTGGT3010.419278451037749No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATACTTTGAAAATGTTGGT2820.3928123694107814No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCGATAACT2760.38445465942331797No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATGAACCCAATAACT2750.38306170775874077No Hit
AATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTT2670.37191809444212287No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATAAACCCAATAACT2520.3510238194734643No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATACTTTGAAAATGTTGGT2440.3398802061568464No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCGATAACT2410.33570135116311467No Hit
GGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTATAT2390.33291544783396015No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTCATATTTTGAAAATGTTGGT2360.32873659284022844No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAGCCCAATAACT2330.3245577378464967No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAGCCCAATAACT2120.2953057528903747No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTTATATTTTGAAAATGTTGGT2080.2897339462320658No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTTATATTTTGAAAATGTTGGT2080.2897339462320658No Hit
TAATACAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTT1790.2493383479593258No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATGAACCCAATAACT1770.2465524446301713No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTAT1730.24098063797186237No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATCTTGAAAATGTTGGT1700.23680178297813068No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCTATATTTTGAAAATGTTGGT1650.22983702465524447No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGGACCCAATAACT1640.22844407299066724No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCCATATTTTGAAAATGTTGGT1530.2131216046803176No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1520.21172865301574034TruSeq Adapter, Index 6 (100% over 50bp)
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAGACCCAATAACT1510.2103357013511631No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTCTGAAAATGTTGGT1500.20894274968658588No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAACCCAATAACTT1420.19779913636996793No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATCTTGAAAATGTTGGT1320.18386961972419558No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAATATGAACCCAATAACT1320.18386961972419558No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAATATAAACCCAATAACT1280.1782978130658866No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATAAACCCAATAACT1220.1699401030784232No Hit
TAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTA1180.16436829642011422No Hit
ACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTATT1110.15461763476807355No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTCTGAAAATGTTGGT1040.1448669731160329No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTATATTTTGAAAATGTTGGTT980.13650926312856942No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATTTTGAAAATGTTGGTTA970.1351163114639922No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTCGAAAATGTTGGT930.12954450480568325No Hit
CAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTATTT890.12397269814737429No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATAAACCCAATAACT870.12118679481821981No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATGAACCCAATAACT860.11979384315364257No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATGAACCCAATAACTTA800.11143613316617912No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTCGAAAATGTTGGT790.11004318150160189No Hit
AAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTAT750.10447137484329294No Hit
AGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTATA730.10168547151413847No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)