FastQCFastQC Report
Thu 16 Jun 2022
EGAF00006153169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006153169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53510
Sequences flagged as poor quality0
Sequence length100
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACT922817.245374696318443No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGT868716.234348719865448No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACT781014.59540272846197No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGT771214.412259390768082No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTT7931.4819659876658569No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTT6631.2390207437862082No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCGATAACT5521.0315828817043542No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTA4670.8727340683984302No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTCATATTTTGAAAATGTTGGT4260.7961128760979257No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTA4120.7699495421416558No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTCATATTTTGAAAATGTTGGT4090.7643431134367408No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAGCCCAATAACT4040.7549990655952158No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATACTTTGAAAATGTTGGT4000.747523827321996No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATGAACCCAATAACT3880.725098112502336No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTTATATTTTGAAAATGTTGGT3320.6204447766772566No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCGATAACT3320.6204447766772566No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGGACCCAATAACT3280.6129695384040367No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCCATATTTTGAAAATGTTGGT3250.6073631096991217No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATAAACCCAATAACT3070.5737245374696318No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATACTTTGAAAATGTTGGT2900.541954774808447No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAGACCCAATAACT2780.5195290599887871No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCTATATTTTGAAAATGTTGGT2670.4989721547374323No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATGAACCCAATAACT2650.49523453560082226No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATAAACCCAATAACT2620.48962810689590736No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2600.4858904877592973TruSeq Adapter, Index 5 (100% over 50bp)
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATCTTGAAAATGTTGGT2520.47094001121285745No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAGCCCAATAACT2510.4690712016445524No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATCTTGAAAATGTTGGT2420.4522519155298075No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAATATGAACCCAATAACT2390.4466454868248926No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTAT2360.44103905811997757No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTCTGAAAATGTTGGT2270.42421977200523264No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATGAACCCAATAACT2200.41113810502709774No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATAAACCCAATAACT2190.4092692954587927No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTTATATTTTGAAAATGTTGGT2180.4074004858904878No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTCGAAAATGTTGGT2150.4017940571855728No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTAT1940.362549056251168No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTCGAAAATGTTGGT1880.35133619884133804No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTCTGAAAATGTTGGT1700.31769762661184825No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAATATAAACCCAATAACT1690.31582881704354326No Hit
AGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTATA1340.2504204821528686No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAACCCAATAACTT1280.2392076247430387No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTATATTTTGAAAATGTTGGTT1160.21678190992337878No Hit
AGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTATA1140.21304429078676884No Hit
AATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTT1120.20930667165015882No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATTTTGAAAATGTTGGTTA1110.20743786208185386No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCATAACTT1090.2037002429452439No Hit
AGTAAGGTTAGTTAAATAAGTTATGGGTTCATATTTTGAAAATGTTGGTT1050.19622500467202392No Hit
AATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTT1030.19248738553541395No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATGAACCCAATAACTTA960.17940571855727902No Hit
CAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTATTT910.17006167071575407No Hit
CAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTATTT900.16819286114744908No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAACTTAT840.15698000373761914No Hit
TAATACAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTT810.15137357503270416No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTGAAAATGTTGGTT790.14763595589609418No Hit
GGGGTGGAGCAAGCGTGACAAGTCCACGTATGACCCGACCGACGATATCG750.14016071762287424No Hit
AGTAAGGTTAGTTAAATAAGTTGGGTTCATATTTTGAAAATGTTGGTTAT740.13829190805456923No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATTTTGAAAATGTTGGTTA720.13455428891795926No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATAAACCCAATAACTTA670.1252102410764343No Hit
AGTAAGGTTAGTTAAATAAGTTATGGGTTTATATTTTGAAAATGTTGGTT640.11960381237151935No Hit
GGGGAACACGATGATATTCACCACACCGCTGGCGTTCAGCGCGTAGGCTT630.11773500280321436No Hit
TAATACAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTT620.11586619323490936No Hit
GGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTATAT600.11212857409829938No Hit
GGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTATAT600.11212857409829938No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCATAACTT580.10839095496168939No Hit
TAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTA580.10839095496168939No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAATATAAACCCAATAACTT570.10652214539338442No Hit
AGTAAGGTTAGTTAAATAAGTTGGGTTTATATTTTGAAAATGTTGGTTAT560.10465333582507941No Hit
TAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTA550.10278452625677444No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)