FastQCFastQC Report
Mon 6 Jun 2022
EGAF00006153171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006153171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76880
Sequences flagged as poor quality0
Sequence length100
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACT1327817.271071800208116No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGT1306716.996618106139437No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACT1076914.00754422476587No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGT1063213.829344432882415No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTT12031.5647762747138398No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTT9491.2343912591050987No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTA8191.065296566077003No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCGATAACT7140.9287200832466181No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTCATATTTTGAAAATGTTGGT6560.8532778355879292No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTA6180.8038501560874091No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAGCCCAATAACT5970.7765348595213319No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTCATATTTTGAAAATGTTGGT5910.7687304890738814No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCGATAACT5350.6958896982310093No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATAAACCCAATAACT5000.6503642039542143No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCCATATTTTGAAAATGTTGGT4810.6256503642039543No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATGAACCCAATAACT4710.6126430801248699No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATACTTTGAAAATGTTGGT4640.603537981269511No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATACTTTGAAAATGTTGGT4610.5996357960457857No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAGCCCAATAACT4590.5970343392299687No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTTATATTTTGAAAATGTTGGT4580.5957336108220603No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGGACCCAATAACT4520.5879292403746098No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTTATATTTTGAAAATGTTGGT4510.5866285119667014No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATGAACCCAATAACT4050.5267950052029137No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTAT4040.5254942767950052No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATCTTGAAAATGTTGGT3850.5007804370447451No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAGACCCAATAACT3840.4994797086368366No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATAAACCCAATAACT3780.4916753381893861No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCTATATTTTGAAAATGTTGGT3710.48257023933402704No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTCGAAAATGTTGGT3690.4799687825182102No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTCGAAAATGTTGGT3670.4773673257023933No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAATATAAACCCAATAACT3670.4773673257023933No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATAAACCCAATAACT3650.47476586888657646No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTCTGAAAATGTTGGT3530.4591571279916753No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAATATGAACCCAATAACT3490.4539542143600416No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTAT3460.45005202913631637No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTCTGAAAATGTTGGT3460.45005202913631637No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATCTTGAAAATGTTGGT2920.3798126951092612No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATGAACCCAATAACT2830.36810613943808534No Hit
AGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTATA2050.2666493236212279No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTATATTTTGAAAATGTTGGTT1920.2497398543184183No Hit
AATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTT1770.23022892819979188No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1710.2224245577523413TruSeq Adapter, Index 4 (100% over 50bp)
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAACCCAATAACTT1680.218522372528616No Hit
AGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTATA1600.20811654526534862No Hit
CAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTATTT1580.20551508844953173No Hit
GGGGTGGAGCAAGCGTGACAAGTCCACGTATGACCCGACCGACGATATCG1290.1677939646201873No Hit
CAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTATTT1280.16649323621227888No Hit
AATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTT1210.15738813735691987No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATTTTGAAAATGTTGGTTA1060.13787721123829344No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCATAACTT1030.13397502601456815No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATGAACCCAATAACTTA990.12877211238293446No Hit
GGGGAACACGATGATATTCACCACACCGCTGGCGTTCAGCGCGTAGGCTT970.12617065556711757No Hit
AGTAAGGTTAGTTAAATAAGTTATGGGTTCATATTTTGAAAATGTTGGTT960.12486992715920915No Hit
TAATACAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTT950.12356919875130072No Hit
TAATACAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTT840.109261186264308No Hit
TAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTA830.10796045785639959No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)