FastQCFastQC Report
Thu 16 Jun 2022
EGAF00006153173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006153173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67045
Sequences flagged as poor quality0
Sequence length100
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACT1128516.83197852188828No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGT1126116.796181669028265No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACT904013.483481243940638No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGT882113.156834961592958No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTT10531.5705869192333508No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTT8161.2170929972406592No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTA7841.1693638600939666No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTA5970.8904467148929822No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTCATATTTTGAAAATGTTGGT5930.8844805727496458No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCGATAACT5530.82481915131628No Hit
GGGGTGGAGCAAGCGTGACAAGTCCACGTATGACCCGACCGACGATATCG5280.7875307629204267No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTCATATTTTGAAAATGTTGGT5210.7770900141695877No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATACTTTGAAAATGTTGGT5040.7517339100604071No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTTATATTTTGAAAATGTTGGT4960.7398016257737341No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCGATAACT4800.7159370572003878No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAGCCCAATAACT4800.7159370572003878No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATGAACCCAATAACT4720.7040047729137147No Hit
GGGGAACACGATGATATTCACCACACCGCTGGCGTTCAGCGCGTAGGCTT4560.6801402043403684No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCCATATTTTGAAAATGTTGGT4180.623461853978671No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATAAACCCAATAACT3780.5638004325453053No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATACTTTGAAAATGTTGGT3750.559325825937803No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTAT3730.5563427548661346No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGGACCCAATAACT3500.5220374375419494No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTCGAAAATGTTGGT3480.5190543664702811No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAGCCCAATAACT3430.5115966887911104No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATGAACCCAATAACT3200.4772913714669252No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATGAACCCAATAACT3190.47579983593109104No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATCTTGAAAATGTTGGT3140.4683421582519203No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCTATATTTTGAAAATGTTGGT3030.4519352673577448No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTTATATTTTGAAAATGTTGGT2960.44149451860690575No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATCTTGAAAATGTTGGT2950.4400029830710717No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAGACCCAATAACT2840.4235960921768961No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATAAACCCAATAACT2830.42210455664106195No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTAT2770.41315534342605714No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTCTGAAAATGTTGGT2600.3877992393168767No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAATATGAACCCAATAACT2430.36244313520769633No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTCGAAAATGTTGGT2190.32664628234767695No Hit
CCTGCCCCAGCACCTGTTTGAATCGCTATGCATCGATCATTTGCAACGCC2190.32664628234767695No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAATATAAACCCAATAACT2170.32366321127600867No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTCTGAAAATGTTGGT2120.31620553359683795No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2120.31620553359683795TruSeq Adapter, Index 3 (100% over 50bp)
TAATACAAAAAAAATATAACCAACATTTTCGAAATATAAACCCAATAACT2110.3147139980610038No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCATAACTT2090.3117309269893355No Hit
CCTATGGTTTCAACCATGTACCGGATGTGTTCTGCCATGCGCTCCTGAAA2090.3117309269893355No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATTTTGAAAATGTTGGTTA1870.27891714520098443No Hit
AGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTATA1830.27295100305764786No Hit
AGTAAGGTTAGTTAAATAAGTTATGGGTTCATATTTTGAAAATGTTGGTT1810.26996793198597957No Hit
CCTCGCTTCCGGCAATACTCGTAAACCATATCAACCAGCTCGCTGACGTT1800.2684763964501454No Hit
CCTGCTTTTCGGGAAAACGGGATCACCACGATGGAACAGGTTAACGCAGG1760.26251025430680885No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATGAACCCAATAACTTA1470.21925572376761876No Hit
AATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTT1420.21179804608844804No Hit
AATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTT1280.1909165485867701No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAACCCAATAACTT1270.18942501305093593No Hit
CAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTTATTT1250.18644194197926764No Hit
CAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTATTT1230.18345887090759938No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTATATTTTGAAAATGTTGGTT1120.16705198001342383No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCATAACTT1020.1521366246550824No Hit
AGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTTATA1000.14915355358341412No Hit
GGGCTGGCGTTCAGCGCGTAGGCTTCGATATCGTCGGTCGGGTCATACGT820.12230591393839957No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATTTTGAAAATGTTGGTTA810.12081437840256544No Hit
GGGGTGGAGCAAGCGTGACAAGTCCACGTATGACCCGACCGACGATACCC810.12081437840256544No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATAAACCCAATAACTTA800.1193228428667313No Hit
AGTAAGGTTAGTTAAATAAGTTATGGGTTTATATTTTGAAAATGTTGGTT780.11633977179506302No Hit
TAATACAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTT780.11633977179506302No Hit
GGGTATCGTCGGTCGGGTCATACGTGGACTTGTCACGCTTGCTCCACCCC720.10739055858005817No Hit
TAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTA720.10739055858005817No Hit
TAATACAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACTT710.10589902304422402No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)