FastQCFastQC Report
Wed 1 Jun 2022
EGAF00006153177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006153177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41980
Sequences flagged as poor quality0
Sequence length100
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGT1362232.44878513577894No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACT1240929.55931395902811No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTT9002.1438780371605524No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACT8582.0438303954263937No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGT8492.0223916150547883No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTAACTAACCTTACT5661.348261076703192No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTCATATTTTGAAAATGTTGGT5591.3315864697474988No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCGATAACT5321.2672701286326824No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTCATATTTTGAAAATGTTGGT5091.2124821343496903No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAGCCCAATAACT4751.1314911862791805No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATACTTTGAAAATGTTGGT4551.083849452120057No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCCATATTTTGAAAATGTTGGT4201.0004764173415912No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATCTTGAAAATGTTGGT3910.9313959028108623No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGGACCCAATAACT3810.9075750357313006No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTA3690.8789899952358265No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTCTGAAAATGTTGGT3630.8646974749880896No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATGAACCCAATAACT3450.8218199142448784No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTCGAAAATGTTGGT3400.8099094807050977No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATGAACCCAATAACT3320.7908527870414483No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATGAACCCAATAACT3190.759885659838018No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAATATGAACCCAATAACT3070.7313006193425441No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTAT1780.4240114340161982No Hit
GGGGTGGAGCAAGCGTGACAAGTCCACGTATGACCCGACCGACGATATCG1730.4121010004764173No Hit
GGGGAACACGATGATATTCACCACACCGCTGGCGTTCAGCGCGTAGGCTT1400.3334921391138637No Hit
AGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTATA1200.28585040495474034No Hit
TAATACAAAAAAAATATAACCAACATTTTTTTTGTATTAAGATCGGAAGA1090.2596474511672225No Hit
AATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTT990.2358265840876608No Hit
TAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTA890.2120057170080991No Hit
CCTATGGTTTCAACCATGTACCGGATGTGTTCTGCCATGCGCTCCTGAAA770.18342067651262506No Hit
TAATACAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTT700.16674606955693186No Hit
CCTGCCCCAGCACCTGTTTGAATCGCTATGCATCGATCATTTGCAACGCC570.13577894235350166No Hit
AGTAAGGTTAGTTAAAAAAGTTATTGGGTTCATATTTTGAAAATGTTGGT570.13577894235350166No Hit
CAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTATTT480.11434016198189614No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCGATAACT450.10719390185802763No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTT450.10719390185802763No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC420.10004764173415913TruSeq Adapter, Index 7 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)