FastQCFastQC Report
Mon 6 Jun 2022
EGAF00006153178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006153178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41980
Sequences flagged as poor quality0
Sequence length100
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACT1355332.284421152929966No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGT1222429.118627918056216No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACT8852.10814673654121No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGT8371.993806574559314No Hit
AGTAAGGTTAGTTAAATATGAACCCAATAACTTATTTAACTAACCTTACT5841.391138637446403No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTT5251.250595521676989No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTCATATTTTGAAAATGTTGGT5251.250595521676989No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCGATAACT5161.2291567413053837No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTCATATTTTGAAAATGTTGGT5101.2148642210576466No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAGCCCAATAACT4821.1481657932348737No Hit
AGTAAGGTTAGTTAAATATAACCAACATTTTCAAAATATGAACCCAATAA4531.0790852787041447No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATGAACCCAATAACT4381.0433539780848022No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGGACCCAATAACT4170.9933301572177228No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCCATATTTTGAAAATGTTGGT3980.9480705097665555No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATGAACCCAATAACT3790.9028108623153883No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAATATGAACCCAATAACT3510.8361124344926154No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATGAACCCAATAACT3490.8313482610767031No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATACTTTGAAAATGTTGGT3330.7932348737494046No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATCTTGAAAATGTTGGT3330.7932348737494046No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTCGAAAATGTTGGT2960.7050976655550262No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTCTGAAAATGTTGGT2940.7003334921391139No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTA2610.6217246307765603No Hit
GGGGTGGAGCAAGCGTGACAAGTCCACGTATGACCCGACCGACGATATCG1600.38113387327298714No Hit
GGGGAACACGATGATATTCACCACACCGCTGGCGTTCAGCGCGTAGGCTT1430.34063839923773226No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTAT1240.29537875178656503No Hit
TAATACAAAAAAAATGTTGGTTATATTTTTTTTGTATTAAGATCGGAAGA1220.2906145783706527No Hit
AATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTT930.22153406383992377No Hit
CCTGCCCCAGCACCTGTTTGAATCGCTATGCATCGATCATTTGCAACGCC750.17865650309671272No Hit
AGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTATA720.1715102429728442No Hit
TAATACAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTT620.14768937589328252No Hit
CCTATGGTTTCAACCATGTACCGGATGTGTTCTGCCATGCGCTCCTGAAA560.1333968556455455No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAACCAACATTTTC460.1095759885659838No Hit
TAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTA460.1095759885659838No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTTATATTTTGAAAATGTTGGT440.10481181515007146No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)