FastQCFastQC Report
Thu 16 Jun 2022
EGAF00006153181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006153181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225
Sequences flagged as poor quality0
Sequence length100
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGT7633.77777777777778No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACT5122.666666666666664No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGT94.0No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTT73.111111111111111No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTA41.7777777777777777No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACT41.7777777777777777No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTAACTAACCTTACT31.3333333333333335No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATGAACCCAATAACT31.3333333333333335No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCCATATTTTGAAAATGTTGGT31.3333333333333335No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGGACCCAATAACT31.3333333333333335No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTCATATTTTGAAAATGTTGGT20.8888888888888888No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATACTTTGAAAATGTTGGT20.8888888888888888No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTCATATTTTGAAAATGTTGGT20.8888888888888888No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATGAACCCAATAACT20.8888888888888888No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTCTGAAAATGTTGGT20.8888888888888888No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTCGAAAATGTTGGT20.8888888888888888No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTAT20.8888888888888888No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATGAACCCAATAACT20.8888888888888888No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAATATGAACCCAATAACT20.8888888888888888No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATACTCTGAAAATGTTGGT20.8888888888888888No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAGCCCAATAACT20.8888888888888888No Hit
CATGATGAGACAGGCCGTTTGAATGTTGACGGGATGAACATAATAAGCAA10.4444444444444444No Hit
GACTTCACGCCAGAATACGAAAGACCAGGTATATGCACAAAATGAGATGC10.4444444444444444No Hit
AGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTATA10.4444444444444444No Hit
AGTCAAAATAATCAGCGTGACATTCAGAAGGGTAATAAGAACGAACCATA10.4444444444444444No Hit
GTTAAATCCAAAACGGCAGAAGCCTGAATGAGCTTAATAGAGGCCAAAGC10.4444444444444444No Hit
ATCATGGCGACCATCCAAAGGATAAACATCATAGGCAGTCGGGAGGGTAG10.4444444444444444No Hit
GTTATTGGGTTCATATTTTGAAAATGTTGGTTATATTTTTTTTGTATTAA10.4444444444444444No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTCCGAAAATGTTGGT10.4444444444444444No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAGCCCGATAACT10.4444444444444444No Hit
AAATGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATTATTTG10.4444444444444444No Hit
CAAACCATGAAACCAACATAAACATTATTGCCCGGCGTACGGGGAAGGAC10.4444444444444444No Hit
GGTGGCGCCATGTCTAAATTGTTTGGAGGCGGTCAAAAAGCCGCCTCCGG10.4444444444444444No Hit
CACATAGAAACCAACAGCCATATAACTGGTAGCTTTAAGCGGCTCACCTT10.4444444444444444No Hit
CAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTATTT10.4444444444444444No Hit
AATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTT10.4444444444444444No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATCTTGAAAATGTTGGT10.4444444444444444No Hit
AACAGGCCACAACCAACCAGAACGTGAAAAAGCGTCCTGCGTGTAGCGAA10.4444444444444444No Hit
AACTGGTAGCTTTAAGCGGCTCACCTTTAGCATCAACAGGCCACAACCAA10.4444444444444444No Hit
AAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTAT10.4444444444444444No Hit
TAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTA10.4444444444444444No Hit
ACGCGGCACAGAATGTTTATAGGTCTGTTGAACACGACCAGAAAACTGGC10.4444444444444444No Hit
AGTAAGGTTAGTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTT10.4444444444444444No Hit
GTAAGGTTAGTTAAATAAGTTATTGGATTCATATTTTGAAAATGTTGGTT10.4444444444444444No Hit
CCAAATGCTTACTCAAGCTCAAACGGCTGGTCAGTATTTTACCAATGACC10.4444444444444444No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGTT10.4444444444444444No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCATAACTT10.4444444444444444No Hit
ATTGTAGCATTGTGCCAATTCATCCATTAACTTCTCAGTAACAGATACAA10.4444444444444444No Hit
AATACAAAAAAAATATAACCAACATTTTCAAAATATGAGCCCAATAACTT10.4444444444444444No Hit
CTACTGTAGACATTTTTACTTTTTATGTCCCTCATCGTCACGTTTATGGT10.4444444444444444No Hit
AGGTAAAAAACGTTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAG10.4444444444444444No Hit
TCGGATATTTCTGATGAGTCGAAAAATTATCTTGATAAAGCAGGAATTAC10.4444444444444444No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCTATATTTTGAAAATGTTGGT10.4444444444444444No Hit
TAATACAAAAAAAATATAACCACATTTTCAAAATATGAACCCAATAACTT10.4444444444444444No Hit
AGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTAAA10.4444444444444444No Hit
TAATACAGAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACT10.4444444444444444No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGTTCATATTTCGAAAATGTTGGT10.4444444444444444No Hit
GGGGAAGGACGTCAATAGTCACACAGTCCTTGACGGTATAATAACCACCA10.4444444444444444No Hit
TAATACAAAAAAAATATAACCAACATTTTTTTTGTATTAAGATCGGAAGA10.4444444444444444No Hit
TTGAATTATGGCGAGAAATAAAAGTCTGAAACATGATTAAACTCCTAAGC10.4444444444444444No Hit
TTGAATGTTGACGGGATGAACATAATAAGCAATGACGGCAGCAATAAACT10.4444444444444444No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)