FastQCFastQC Report
Mon 6 Jun 2022
EGAF00006153182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006153182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225
Sequences flagged as poor quality0
Sequence length100
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACT8035.55555555555556No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGT5223.11111111111111No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAAACCCAATAACT94.0No Hit
AGTAAGGTTAGTTAAATATAACCAACATTTTCAAAATATGAACCCAATAA41.7777777777777777No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTTATATTTTGAAAATGTTGGT31.3333333333333335No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGCTCATATTTTGAAAATGTTGGT31.3333333333333335No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTCGAAAATGTTGGT31.3333333333333335No Hit
AGTAAGGTTAGTTAAATATGAACCCAATAACTTATTTAACTAACCTTACT31.3333333333333335No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTCCATATTTTGAAAATGTTGGT31.3333333333333335No Hit
AGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTATA20.8888888888888888No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATACTTTGAAAATGTTGGT20.8888888888888888No Hit
GTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTT20.8888888888888888No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATGAACCCAATAACT20.8888888888888888No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATCTTGAAAATGTTGGT20.8888888888888888No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAGATATGAACCCAATAACT20.8888888888888888No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTCTGAAAATGTTGGT20.8888888888888888No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAGTATGAACCCAATAACT20.8888888888888888No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAATATGAACCCAATAACT20.8888888888888888No Hit
TAATACAAAAAAAATATAACCAACATTTTCAGAGTATGAACCCAATAACT20.8888888888888888No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCGATAACT20.8888888888888888No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGGACCCAATAACT20.8888888888888888No Hit
ATGGTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTGGATTACTATCT10.4444444444444444No Hit
TAATACAAAAAAAATATAACCAGCATTTTCAAAATATGAACCCAATAACT10.4444444444444444No Hit
GACAGGCTCATGCTGATGGTTGGTTTATCGTTTTTGACACTCTCACGTTG10.4444444444444444No Hit
AAGCGGAGCAGTCCAAATGTTTTTGAGATGGCAGCAACGGAAACCATAAC10.4444444444444444No Hit
TGAGTCCGATGCTGTTCAACCACTAATAGGTAAGAAATCATGAGTCAAGT10.4444444444444444No Hit
AGATAATCACGAGTATCCTTTCCTTTATCAGCGGCAGACTTGCCACCAAG10.4444444444444444No Hit
TTTCAGACTTTTATTTCTCGCCATAATTCAAACTTTTTTTCTGATAAGCT10.4444444444444444No Hit
GGAAGGTTAGTTAAATAAGTTATTGGGCTCATATTTTGAAAATGGTGGTT10.4444444444444444No Hit
ATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTA10.4444444444444444No Hit
CAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTATTT10.4444444444444444No Hit
AGTAAGGTTAGTTAAATAAGTTATGGGTTCATATTTTGAAAATGTTGGTT10.4444444444444444No Hit
AAGCCTGAATGAGCTTAATAGAGGCCAAAGCGGTCTGGAAACGTACGGAT10.4444444444444444No Hit
TAATACAAAAAAAATATAACCAACATTTTCGGAATATGAACCCAATAACT10.4444444444444444No Hit
AGCTGGTTCTCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTTTACAG10.4444444444444444No Hit
CGTTGGCTGACGACCGATTAGAGGCGTTTTATGATAATCCCAATGCTTTG10.4444444444444444No Hit
TAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTTGGTTA10.4444444444444444No Hit
GCGCCGTGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGCTTAATCA10.4444444444444444No Hit
AGTAAGGTTAGTTAAATAAGTTATCGGGCTCATATTTTGAAAATGTTGGT10.4444444444444444No Hit
CACACCAGAAGCAGCATCAGTGACGACATTAGAAATATCCTTTGCAGTAG10.4444444444444444No Hit
AGTAAGGTTAGTTAAAAAAATATAACCAACATTTTCAAAATATGAACCCA10.4444444444444444No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAACATGTTGGT10.4444444444444444No Hit
TGACACCCTCCCAATTGTATGTTTTCATGCCTCCAAATCTTGGAGGCTTT10.4444444444444444No Hit
TAATACAAAAAAAATATAACCAACATTTTCGAAATATGAACCCGATAACT10.4444444444444444No Hit
ATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAAGGCTAATGATTCACA10.4444444444444444No Hit
TACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACTTAT10.4444444444444444No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATAGACCCAATAACT10.4444444444444444No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTGCATATTTTGAAAATGTTGGT10.4444444444444444No Hit
TAATACAAAAAAAATGTTGGTTATATTTTTTTTGTATTAAGATCGGAAGA10.4444444444444444No Hit
TAATCCACTGTTCACCATAAACGTGACGATGAGGGACATAAAAAGTAAAA10.4444444444444444No Hit
CCTGCTTATCACCTTCTTGAAGGCTTCCCATTCATTCAGGAACCGCCTTC10.4444444444444444No Hit
AAATCAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGACTTAGTTCAT10.4444444444444444No Hit
ACTCCAGCTTCTTCGGCACCTGTTTTACAGACACCTAAAGCTACATCGTC10.4444444444444444No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAATCCAATAACT10.4444444444444444No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTGTATATTTTGAAAATGTTGGT10.4444444444444444No Hit
AGTAAGGTTAGTTAAATAAGTTATTGGGTTCATATTTTGAAAATGTGGTT10.4444444444444444No Hit
AGTAAGGTTAGTTAAATATAACCAACTTTTTCAAAATATGAACCCAATAA10.4444444444444444No Hit
CTGACGGTTATTTCCTAGACAAATTAGAGCCAATACCATCAGCTTTACCG10.4444444444444444No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAACCCAATAACA10.4444444444444444No Hit
TAATACAAAAAAAATATAACCAACATTTTCAAAATATGAGCCCAATAACT10.4444444444444444No Hit
TTTACGAATTAAATCGAAGTGGACTGCTGGCGGAAAATGAGAAAATTCGA10.4444444444444444No Hit
TTCAAGAAGTCCTTTACCAGCTTTAGCCATAGCACCAGAAACAAAACTAG10.4444444444444444No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)