FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006153466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006153466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25359851
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT964200.380207281186313No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT687070.2709282479617092No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA556740.21953599017596753No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG544940.21488296599218976No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC428900.16912559935781957No Hit
CCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGG427580.16860509156776984No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC392840.1549062729114615No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA390870.15412945446722065No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG370320.14602609455394672No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA351210.13849056132072698No Hit
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAA339590.13390851547195604No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTT324100.12780043542053934No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG313820.1237467838434855No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC307580.12128620156325051No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG305310.12039108589399834No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC301400.11884927872801776No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC300870.11864028696383112No Hit
CCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGTC300810.11861662751882888No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG300060.11832088445630064No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA294690.1162033641285984No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG294300.11604957773608371No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCA289870.1143027220467502No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC284240.1120826774573715No Hit
CCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAAC282000.11119939151062047No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG278700.10989812203549619No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG269780.10638075121182691No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC262470.10349824216238494No Hit
CCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCG254590.10039096838542151No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)