FastQCFastQC Report
Sun 24 Jul 2022
EGAF00006153649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006153649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53247588
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA4278280.8034692576121945No Hit
TGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTA2740880.5147425644894938No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC2286910.42948612057319857TruSeq Adapter, Index 3 (100% over 50bp)
GGACTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTA1984120.37262157301848114No Hit
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT1972850.3705050452238325No Hit
GCCGGGGCTTCTCCCGCCTTTTTTCCCGGCGGCGGGAGAAGTAGATTGAA1457380.2736987823748937No Hit
CCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAGCTAAG1282100.2407808594071904No Hit
GCAGCTTCAAACCTGCCGGGGCTTCTCCCGCCTTTTTTCCCGGCGGCGGG1247270.23423971805070307No Hit
CAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAG1211920.22760091968860638No Hit
GGGTGAGGTAAAATGGCTGAGTGAAGCATTGGACTGTAAATCTAAAGACA1103130.20716994730352856No Hit
CCGGGGCTTCTCCCGCCTTTTTTCCCGGCGGCGGGAGAAGTAGATTGAAG988690.18567789399211848No Hit
TGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAGCTCCGAGGTGA970130.1821922900996004No Hit
CTGCCGGGGCTTCTCCCGCCTTTTTTCCCGGCGGCGGGAGAAGTAGATTG904620.16988938541216178No Hit
CGGGGCTTCTCCCGCCTTTTTTCCCGGCGGCGGGAGAAGTAGATTGAAGC783830.14720478982071453No Hit
CTGAACGCAAATCAGCCACTTTAATTAAGCTAAGCCCTTACTAGACCAAT748110.14049650474308809No Hit
CCTCTTTTTACCAGCTCCGAGGTGATTTTCATATTGAATTGCAAATTCGA731390.13735645640887997No Hit
CCTTACTAGACCAATGGGACTTAAACCCACAAACACTTAGTTAACAGCTA658910.12374457224240842No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCT626530.11766354562388816No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC626180.11759781494703571TruSeq Adapter, Index 3 (100% over 50bp)
CAGCTTCAAACCTGCCGGGGCTTCTCCCGCCTTTTTTCCCGGCGGCGGGA606790.11395633544941042No Hit
GTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGTGGGTTTAAGTCCC599870.11265674606707068No Hit
CTTTAATTAAGCTAAGCCCTTACTAGACCAATGGGACTTAAACCCACAAA599270.1125440649067522No Hit
CTCCGAGGTGATTTTCATATTGAATTGCAAATTCGAAGAAGCAGCTTCAA597580.11222667963852184No Hit
CTTAAACCCACAAACACTTAGTTAACAGCTAAGCACCCTAATCAACTGGC596050.11193934267970974No Hit
GTGATTTTCATATTGAATTGCAAATTCGAAGAAGCAGCTTCAAACCTGCC587300.11029607575839867No Hit
ACTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATA585130.10988854556191352No Hit
CCGAGGTGATTTTCATATTGAATTGCAAATTCGAAGAAGCAGCTTCAAAC582820.1094547230946874No Hit
CCCTAATCAACTGGCTTCAATCTACTTCTCCCGCCGCCGGGAAAAAAGGC581450.10919743444529356No Hit
CGCAAATCAGCCACTTTAATTAAGCTAAGCCCTTACTAGACCAATGGGAC576920.10834669168488908No Hit
GGTTAGGCCTCTTTTTACCAGCTCCGAGGTGATTTTCATATTGAATTGCA568520.10676915544043047No Hit
AGTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGTGGGTTTAAGTCC563350.10579821944235296No Hit
GCTGAGTGAAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTT561210.10539632330388374No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT547900.10289667956415229No Hit
CAGCTAAGCACCCTAATCAACTGGCTTCAATCTACTTCTCCCGCCGCCGG537830.10100551409014057No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)