FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006153650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006153650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53247588
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA4483990.842101993427383No Hit
TGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTA2811320.5279713327108826No Hit
GGACTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTA2042850.383651180594321No Hit
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT2028160.38089237018585703No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT2010470.37757015397580074Illumina Single End PCR Primer 1 (100% over 50bp)
CCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAGCTAAG1326850.24918499594760987No Hit
GCCGGGGCTTCTCCCGCCTTTTTTCCCGGCGGCGGGAGAAGTAGATTGAA1287730.24183818429484544No Hit
CAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAG1257260.23611585937000565No Hit
GGGTGAGGTAAAATGGCTGAGTGAAGCATTGGACTGTAAATCTAAAGACA1137520.2136284558091157No Hit
GCAGCTTCAAACCTGCCGGGGCTTCTCCCGCCTTTTTTCCCGGCGGCGGG1091500.20498581081268885No Hit
TGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAGCTCCGAGGTGA1008780.18945083484344868No Hit
CCGGGGCTTCTCCCGCCTTTTTTCCCGGCGGCGGGAGAAGTAGATTGAAG848710.15938937928981872No Hit
CTGCCGGGGCTTCTCCCGCCTTTTTTCCCGGCGGCGGGAGAAGTAGATTG789150.14820389610887164No Hit
CTGAACGCAAATCAGCCACTTTAATTAAGCTAAGCCCTTACTAGACCAAT786240.14765739248132703No Hit
CCTCTTTTTACCAGCTCCGAGGTGATTTTCATATTGAATTGCAAATTCGA739600.1388983102859044No Hit
CGGGGCTTCTCCCGCCTTTTTTCCCGGCGGCGGGAGAAGTAGATTGAAGC678480.12741985608812928No Hit
CCTTACTAGACCAATGGGACTTAAACCCACAAACACTTAGTTAACAGCTA668210.12549113022734476No Hit
CTTTAATTAAGCTAAGCCCTTACTAGACCAATGGGACTTAAACCCACAAA646660.12144399855257293No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG630870.11847860601685845Illumina Single End PCR Primer 1 (100% over 50bp)
ACTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATA614470.11539865430148687No Hit
CTCCGAGGTGATTTTCATATTGAATTGCAAATTCGAAGAAGCAGCTTCAA612990.11512070743936796No Hit
CGCAAATCAGCCACTTTAATTAAGCTAAGCCCTTACTAGACCAATGGGAC612210.11497422193095395No Hit
GGTTAGGCCTCTTTTTACCAGCTCCGAGGTGATTTTCATATTGAATTGCA604220.1134736844793796No Hit
CCGAGGTGATTTTCATATTGAATTGCAAATTCGAAGAAGCAGCTTCAAAC598050.11231494654743798No Hit
CTTAAACCCACAAACACTTAGTTAACAGCTAAGCACCCTAATCAACTGGC595100.11176093084253882No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCT588030.11043317117011948No Hit
GCTGAGTGAAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTT582450.10938523637915769No Hit
GTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGTGGGTTTAAGTCCC577660.10848566511594854No Hit
GTGATTTTCATATTGAATTGCAAATTCGAAGAAGCAGCTTCAAACCTGCC568640.10679169167249415No Hit
AGTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGTGGGTTTAAGTCC564600.10603297185968312No Hit
CAGCTTCAAACCTGCCGGGGCTTCTCCCGCCTTTTTTCCCGGCGGCGGGA535250.10052098510077113No Hit
CTTTAACTCCACCATTAGCACCCAAAGCTAAGATTCTAATTTAAACTATT534380.10035759741830937No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT532850.10007026045949724No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)