FastQCFastQC Report
Thu 21 Jul 2022
EGAF00006153930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006153930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50478512
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC2485640.4924154658124629Illumina Single End PCR Primer 1 (96% over 31bp)
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT992740.19666586051506432No Hit
GCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA546140.10819257112808713No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)