FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006154204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006154204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114473428
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC1720440.1502916467217178Illumina Single End PCR Primer 1 (96% over 31bp)
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC1665780.1455167394829829No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT1177780.10288675901275535No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA1169670.1021782976569899No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)