FastQCFastQC Report
Fri 22 Jul 2022
EGAF00006158395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503712955
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGATCCACTGCCGCTGAACCTTGGTGGGACCCCAATTTCTAAAGTTG27028730.5365899314620566No Hit
CGGAAAACTATCATATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCT15922180.3160962973445859No Hit
CTGGTTTTTGCTGATACCAGGCCAACCAGGTACTGATAGTCTGACTGGCC15168310.3011300354583892No Hit
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAA14899880.295801008334201No Hit
CTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGC14018120.2782958004326095No Hit
CTCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGG13162190.26130338458338837No Hit
CCAATTTCTAAAGTTGACGCCTCATAGATCAGGAGTTTAGGGGCTTTCCC12636470.2508664880378151No Hit
GGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTC12621650.250572272853296No Hit
CTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTT12126690.2407460415625006No Hit
CCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACT11637670.23103773457643154No Hit
GGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTT9589680.19037985632114623No Hit
GGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTC8735200.17341622670792733No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG8397030.16670268089491563TruSeq Adapter, Index 8 (97% over 35bp)
GTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT8079840.16040564213799108No Hit
GCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGC7984080.1585045594072521No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC7927580.1573828888319936No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7882850.15649488308276685No Hit
CGGCAAGTGATGGTGACTCTGTCTCCCACAGATGCAGACACGGTGGAAGG7783670.15452590454021578No Hit
CTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTG7782660.154505853437897No Hit
GTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTG7751150.15388029875070416No Hit
GGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTG7631230.15149957777043874No Hit
CCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGG7132450.14159750963720993No Hit
CTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCT6826420.13552202563461963No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6780000.13460046903101788No Hit
CAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCG6656090.13214053627030498No Hit
CATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCC6580120.13063233602955476No Hit
GCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAG6500080.12904333580223284No Hit
CTCCAGCTTGGTTCCCTGGGCAAAAGTGTACGGAAAACTATCATATTGTT6309110.1252520892578592No Hit
CTGGGCAAAAGTGTACGGAAAACTATCATATTGTTGGCAGTAATAAGTTG6283830.12475021612259307No Hit
CGCTGAACCTTGGTGGGACCCCAATTTCTAAAGTTGACGCCTCATAGATC6276930.12461323334437566No Hit
CCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGG6169810.1224866253439918No Hit
GGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGA6153630.12216541065536025No Hit
CCGGCAAGTGATGGTGACTCTGTCTCCCACAGATGCAGACACGGTGGAAG5899070.11711173876796557No Hit
CAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACT5857880.11629401113973732No Hit
CAGGAGTTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCCAACCAGG5844950.11603731732490383No Hit
CGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCT5391260.10703040186846098No Hit
CCACAGTTCGTTTGATCTCCAGCTTGGTTCCCTGGGCAAAAGTGTACGGA5382830.10686304464811709No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA5308980.10539693186965979No Hit
CAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGG5109630.10143932073376988No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)