FastQCFastQC Report
Sat 30 Jul 2022
EGAF00006158396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503712955
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17361240.34466534615930217No Hit
CACCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCACTCTCACCATC16494920.3274666620396928No Hit
CCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCACTCTCACCATCAG14282500.28354442462175705No Hit
GTTTTCCGTACACTTTTGCCCAGGGAACCAAGCTGGAGATCAAACGAACT12691840.2519657252015684No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC11105950.22048172257153878No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10642550.2112820385967639No Hit
CACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT10516370.20877704048727513No Hit
CTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTT10473180.20791960770594037No Hit
GTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATT10304860.20457802202049777No Hit
CCTAAACTCCTGATCTATGAGGCGTCAACTTTAGAAATTGGGGTCCCACC9317420.1849747938287591No Hit
CATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGC8653590.1717960579354168No Hit
CTAAACTCCTGATCTATGAGGCGTCAACTTTAGAAATTGGGGTCCCACCA8257830.1639392022387036No Hit
ACCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCACTCTCACCATCA7801090.15487173642377333No Hit
CTTTTGCCCAGGGAACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA7696480.1527949583905381No Hit
GTCAGACTATCAGTACCTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAA7631820.15151129079060516No Hit
CACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGT7304540.1450139395362583No Hit
GCAAAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGAGGCGTCAACTT7301690.14495735969308154No Hit
GTGACATCCAGATGACCCAGTCTCCTTCCACCGTGTCTGCATCTGTGGGA7118110.14131282368943637No Hit
CCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAG6997900.1389263454619705No Hit
CGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTG6825730.13550832735679788No Hit
CCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC6732610.13365965542815947No Hit
GCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACCGTGTCTGCATC6498350.12900899084479572No Hit
CTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGT6450520.1280594421082539No Hit
TGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGT6423980.12753255472653072No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC6414610.12734653608422677Illumina Single End PCR Primer 1 (96% over 31bp)
TGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTC6293900.12495013156848427No Hit
CAGGGAACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT6153020.12215330058366278No Hit
TGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCA5983960.11879702399157076No Hit
CAGCTCAACACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGC5766890.11448762519915733No Hit
CAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTG5628050.11173129347050445No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT5320240.10562047188164933No Hit
CGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACC5169630.10263047532696475No Hit
GAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5158210.10240375890272667No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)