FastQCFastQC Report
Sat 30 Jul 2022
EGAF00006158402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504931907
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT10873190.21533972896666245Illumina Single End PCR Primer 1 (100% over 50bp)
CTACACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCCTGAAAA9940530.1968687235286955No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT7915330.1567603451132273No Hit
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT7595090.1504181038018657No Hit
CGCGGTCGTAAGGGCTGAGGATTTTTGGTCCGCACGCTCCTGCTCCTGAC7474930.1480383769845624No Hit
CCTACACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCCTGAAA5993820.11870551091951495No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG5853520.11592691843892526No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)