FastQCFastQC Report
Sat 30 Jul 2022
EGAF00006158432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517713053
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC16727080.3230955816754344Illumina Single End PCR Primer 1 (96% over 31bp)
GCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA6362110.12288873079659439No Hit
TGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTA5396120.10422993912807527No Hit
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT5243620.10128429193768076No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)