FastQCFastQC Report
Fri 22 Jul 2022
EGAF00006158437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493110339
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTC20823150.4222817562947103No Hit
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAA15483510.3139968638945938No Hit
GTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT14441830.2928721800740828No Hit
GTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGT14020500.28432784492884056No Hit
CCCCTTTCCAACTGCCCTACACTATCTTTCACACAGTAATACAGGCCCGT12802640.2596303299168911No Hit
CTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGC12791810.25941070361536267No Hit
GCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTAG12452310.2525258347909026No Hit
GTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTG11713570.2375446035821204No Hit
GTCCAGTCGTGTCCCCTGGCCGAAGGTGACCGGAGGCCTTTGTAGAGTTT11610920.2354629193852697No Hit
CCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACT11030420.22369070627010318No Hit
GGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTC10746390.2179307378099813No Hit
GTGGGGACTTCCCTGGCTTGTGCAGGTACCAATCCAAATAGTTGTATGCA10657580.2161297210197006No Hit
ATCAGGAGTTGTGGGGACTTCCCTGGCTTGTGCAGGTACCAATCCAAATA10485150.21263293771660302No Hit
GCCATGTAGATCAGGAGTTGTGGGGACTTCCCTGGCTTGTGCAGGTACCA10404730.21100206540183697No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC9713130.19697680684809166No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9705270.1968174104741292No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC9236440.1873098020765693No Hit
GTGGCGGTTAGCGGGGTCTTGGACTCGGGGTAGGCAGCAGTGCAAATGAA8943560.181370360599963No Hit
GGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCA8913870.18076826411867222No Hit
GGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATGTCCAGTCG8775250.1779571285768559No Hit
CCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGAG8595950.17432102554231782No Hit
CTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAG8224150.1667811309062818No Hit
GCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCA7413790.15034748642737342No Hit
GTAAAATCTGAGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGA7378160.1496249300909507No Hit
CTCCACTCTGCTGATTTTCAGTGTAAAATCTGAGCCTGATCCACTGCCAC7355880.1491731042370215No Hit
GGCCGGTCGGTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGG7310630.14825545971770832No Hit
GGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGT7103690.1440588330475058No Hit
GGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCT6764970.13718978218382072No Hit
CTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGATGACGGA6715540.1361873696183036No Hit
CTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTT6651050.1348795487332096No Hit
CAGGAGTTGTGGGGACTTCCCTGGCTTGTGCAGGTACCAATCCAAATAGT6452950.13086219228512264No Hit
GTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGG6305910.1278803038846849No Hit
GTTCGTTTAATGTCCAGTCGTGTCCCCTGGCCGAAGGTGACCGGAGGCCT6269940.1271508525397193No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA6253600.126819486540922No Hit
GCCACAGTTCGTTTAATGTCCAGTCGTGTCCCCTGGCCGAAGGTGACCGG6142560.12456765786855667No Hit
CCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGAGCC6012750.12193518416574915No Hit
GTGGAGTTGAGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGGATTC5991320.12150059583317721No Hit
GTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAG5877540.11919320150373079No Hit
CCTGGTTTCAAATTCATTGGTAATAAATATGCTAACTTTCTGAATCAAAA5822580.11807864365220701No Hit
GTGGAGTTGAGGGAGATGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGC5719300.11598418341011503No Hit
GCCGGACCCAGTGCATGGCATAATCATCAAAGGTGAATCCAGAGGCAACA5693370.11545833761153403No Hit
GCCAGGCTCTACCAACCCTCCCCCAGACTCCACCAGTTGGACCTCACACT5615790.11388505889753814No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA5599140.11354740627330469No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT5568890.11293395330735502No Hit
GTCAGGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCT5519850.11193944972222535No Hit
GCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATGTCCAGT5504700.11163221625332825No Hit
CTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTT5376810.10903867906935125No Hit
GCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAA5290530.10728896925440454No Hit
CAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCG5246310.10639221255508902No Hit
CTCGGCTGCCACGCGCAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCT5229510.10605151801532194No Hit
CCAGCTGATACCTGAAACCCACTCCAGGCCCTTCCCTGGAGCTAGCCGGA5229210.10604543418425466No Hit
GGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCG5220040.10585947174796512No Hit
GTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATG5207090.10559685304022798No Hit
GTATGCATCACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGG5175190.10494993900340831No Hit
GTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAG5161920.1046808308758661No Hit
TGTAGATCAGGAGTTGTGGGGACTTCCCTGGCTTGTGCAGGTACCAATCC5077100.10296072903878009No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5064900.10271331990871113No Hit
GTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCC5054230.10249693831708526No Hit
CCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGAGC4996810.10133249305080987No Hit
GTTCATTTGCAGCTGCACGGAGTTCTTGGCGTTGTCTCTAGAGATGGTGA4995160.10129903197993989No Hit
GCTGATTTTCAGTGTAAAATCTGAGCCTGATCCACTGCCACTGAACCTGT4952300.10042985531479598No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)