FastQCFastQC Report
Fri 29 Jul 2022
EGAF00006158438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493110339
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG20458960.4148961881734141No Hit
CTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTG18922920.38374616193151856No Hit
GGAGCCCCAGCCCTGAGATTCCCAGGTGTTTCCATTCAGTGATCAGCACT17861280.3622167005506652No Hit
CTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT14696400.29803471632339873No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC13349880.2707280489610663No Hit
CTGCATTCAAACTCTACAAAGGCCTCCGGTCACCTTCGGCCAGGGGACAC11088730.22487320023521143No Hit
CTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTT10002120.20283736131519237No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9503240.192720355839061No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9325670.1891193362303442No Hit
CTCAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGG8875490.17998993933079954No Hit
CCCCAGCCCTGAGATTCCCAGGTGTTTCCATTCAGTGATCAGCACTGAAC8789500.1782461105525512No Hit
CCCAGCCCTGAGATTCCCAGGTGTTTCCATTCAGTGATCAGCACTGAACA8571070.17381647315247226No Hit
GTCAAGATGAAGAACCATTTGCTTTTCTGGGGAGTCCTGGCGGTTTTTAT8100490.1642733757403533No Hit
TGATGACTCAGTCTCCACTCTCCCTGTCCGTCATCCCTGGAGAGCCGGCC8073870.1637335371303176No Hit
GTGATGACTCAGTCTCCACTCTCCCTGTCCGTCATCCCTGGAGAGCCGGC7501250.15212112597785138No Hit
CACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGT7338750.14882571748308038No Hit
GTCAGAGCCTCCTGCATAGTGATGCATACAACTATTTGGATTGGTACCTG7195030.14591115681312047No Hit
AAATGATTTAGGCTATGCGGACTCTGTGAAGGGCCGATTCACCATCTCTA7178410.14557411257199374No Hit
CCTCAAGTGGGAAGAGCGCTGTTCAAGGACCACCTGAGCGTGACCTCTGT6996530.14188568859027714No Hit
GTCCCCACAACTCCTGATCTACATGGCTTCTAATCGGGCCTCCGGGGTCC6895570.13983827664177206No Hit
GTCTGGGGCCAAGGGACCACGGTCACCGTCACCTCAGCATCCCCGACCAG6597880.13380129107372052No Hit
CTGACAGGTTCAGTGGCAGTGGATCAGGCTCAGATTTTACACTGAAAATC6523280.13228844508165952No Hit
GCTGAGGATGTTGGGGTTTATTACTGCATTCAAACTCTACAAAGGCCTCC6494790.1317106839246378No Hit
GCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATA6483720.13148619055825556No Hit
CTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCCCT6457040.13094513518200637No Hit
GTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCCCC6420190.1301978379325768No Hit
CACGACTGGACATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC6400640.12980137494135974No Hit
CACGAATCCCAGCCAGGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTC6396210.12971153703593305No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT6355720.12889042263622058No Hit
CGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACC6278240.12731917186591377No Hit
GGGACCACGGTCACCGTCACCTCAGCATCCCCGACCAGCCCCAAGGTCTT6116550.12404018971502442No Hit
GACAGGTTCAGTGGCAGTGGATCAGGCTCAGATTTTACACTGAAAATCAG6107340.12385341610125923No Hit
GTTGGATTTTCCTTTTGGCTGTTTTAAAAGGTGTCCAGTGTGAGGTCCAA6060480.12290312168855173No Hit
CCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTG5989920.12147220462152995No Hit
GAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5985640.12138540863163691No Hit
CACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGA5974040.12115016716370249No Hit
GTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGG5948070.12062351018764586No Hit
CCCACAACTCCTGATCTACATGGCTTCTAATCGGGCCTCCGGGGTCCCTG5936070.12038015694495506No Hit
GGGGACACGACTGGACATTAAACGAACTGTGGCTGCACCATCTGTCTTCA5924340.12014227915022482No Hit
CTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGT5846440.11856251101642384No Hit
CCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAG5792100.11746052641577244No Hit
GTCCAACTGGTGGAGTCTGGGGGAGGGTTGGTAGAGCCTGGCAGGTCCCT5749230.11659114695625962No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCC5707050.11573576030820153No Hit
AGAGGACTCACCATGGAGTGGGGACTGAGTTGGATTTTCCTTTTGGCTGT5672110.11502719678323353No Hit
GCCCCAGCCCTGAGATTCCCAGGTGTTTCCATTCAGTGATCAGCACTGAA5668470.11495337963295067No Hit
CTTCGGCCAGGGGACACGACTGGACATTAAACGAACTGTGGCTGCACCAT5648390.11454616854018143No Hit
GGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCT5531770.11218118060996485No Hit
AGGAGCCCCAGCCCTGAGATTCCCAGGTGTTTCCATTCAGTGATCAGCAC5490330.11134080074520604No Hit
CCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCG5483510.11120249498561009No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG5479890.11112908342406505No Hit
GGGGAAGTCGTCTTCACTATCATTATTACGGTATGGACGTCTGGGGCCAA5475520.11104046228485184No Hit
CCTGCACAAGCCAGGGAAGTCCCCACAACTCCTGATCTACATGGCTTCTA5472570.11098063794602367No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGG5465940.11084618527943703No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGT5457150.11066792902916603No Hit
CTCTACAAAGGCCTCCGGTCACCTTCGGCCAGGGGACACGACTGGACATT5365470.10880871025500848No Hit
GTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGA5326250.10801335074014744No Hit
GTTGGGGTTTATTACTGCATTCAAACTCTACAAAGGCCTCCGGTCACCTT5311180.10770773962620159No Hit
CTTCACCTGGACGCCCTCAAGTGGGAAGAGCGCTGTTCAAGGACCACCTG5245630.1063784225380032No Hit
CCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCA5238750.10623890001219383No Hit
CTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGTCC5166530.10477431907993315No Hit
CCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGC5118320.10379664742742292No Hit
GAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTC4996900.10133431820013006No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)