FastQCFastQC Report
Wed 13 Jul 2022
EGAF00006158459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481575160
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG21626000.44906801256111306TruSeq Adapter, Index 8 (97% over 35bp)
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT13411200.27848612457502997No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA11877170.24663169919312283No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC8386110.17413917279288244No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA7920210.1644646704784358No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT7451360.15472891085163112No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA7024120.1458571908069345No Hit
CAGGGTGTTGGCTTTACACCAACGAAAAAGAAAAACAGATGAATAAGCTT6674660.13860058728942748No Hit
CTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCA6265070.13009537285934764No Hit
CTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTTGC6245550.129690036338253No Hit
ATTTTTTATTAATTGAAGCACAGAGAAAAGAGGCAAAATGATTAAAGAAA6066640.12597493608266672No Hit
CTTGATTTGTCTCTCCTTCTCAGAAAGTGTGCATATATCTGCATGGAAGG5447370.11311567648132018No Hit
AGGGTGTTGGCTTTACACCAACGAAAAAGAAAAACAGATGAATAAGCTTT5134520.10661928659277194No Hit
CTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCAT5086710.10562650282875886No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)