FastQCFastQC Report
Wed 13 Jul 2022
EGAF00006158460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481575160
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC21081090.437752852535002Illumina Single End PCR Primer 1 (96% over 31bp)
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC8799690.18272724033357535No Hit
CAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCCAAGGCAA7232730.15018901722422726No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC6810470.14142070782886723No Hit
CACATTTAAAAGCATCTCAGCCTACCATGAGAATAAGAGAAAGAAAATGA5653970.11740576486544696No Hit
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT5116580.10624675907287245No Hit
CACAAGCCCAAGGCAACAAAAGAGCAACTGAAAGCTGTTATGGATGATTT5063320.10514080501992668No Hit
CTGAAAGCTGTTATGGATGATTTCGCAGCTTTTGTAGAGAAGTGCTGCAA5011330.10406122275908083No Hit
GGATGATTTCGCAGCTTTTGTAGAGAAGTGCTGCAAGGCTGACGATAAGG4937390.10252584456390981No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)