FastQCFastQC Report
Fri 22 Jul 2022
EGAF00006158485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476921306
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTGACTGGCCCTACAGGCGAGGGTGGCTCTTTCCCCTGGAGACAAAGA21278890.4461719309306765No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC14871980.3118329966160078No Hit
CTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGC14153730.2967728600491587No Hit
GGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTC14135690.29639460058007977No Hit
CCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACT14032480.29423051189916855No Hit
CTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACTCACTGCCTG11855490.2485837778864088No Hit
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAA11190150.2346330486648462No Hit
GTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT10803680.22652961534916205No Hit
GGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTC10792700.22629938868782684No Hit
CTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTT9922610.2080554983634973No Hit
GTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGT9316570.1953481608557031No Hit
CCCTGATGGATGCAGCATAGATGAGGAGTCTGGGAGCCTGGCCAGGTTTC9299050.1949808046529169No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8721190.1828643403069101No Hit
CCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGG8689310.18219588621188587No Hit
CTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCTGGGATAGA8406670.17626954162538505No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC8193370.1717971056633817No Hit
GTGAGAGTGAAGTCTGTCCCAGACTCACTGCCTGAGAACCTGTCTGGGAT8041040.1686030776742023No Hit
CTGGCTGTTCCTAACACTCTGACTGGCCCTACAGGCGAGGGTGGCTCTTT8040970.16860160992681672No Hit
CTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCCCCCAGGAGTTCAG8033200.1684386899670194No Hit
CTGGAGACAAAGACTGGGTGGCTGGAGACTGCGTCAACACAATTTCTCCG7605960.15948039863834473No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG7591150.15916986522719956No Hit
GTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTG7572530.15877944442264025No Hit
CTGGGATGCCAGTGGCCCTGATGGATGCAGCATAGATGAGGAGTCTGGGA7559670.15850979826009282No Hit
CTAACACTCTGACTGGCCCTACAGGCGAGGGTGGCTCTTTCCCCTGGAGA7180570.15056089777628848No Hit
CTGCAAAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC6776270.14208360823368205No Hit
CTGAGAACCTGTCTGGGATGCCAGTGGCCCTGATGGATGCAGCATAGATG6744240.14141200896568876No Hit
CGGGGAAGTAGTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAG6722690.14096015244913382No Hit
GCTGTTCCTAACACTCTGACTGGCCCTACAGGCGAGGGTGGCTCTTTCCC6685260.1401753269542544No Hit
CAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCG6659730.13964001851492036No Hit
CTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGCT6658420.13961255067099057No Hit
CTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTG6584440.1380613513626502No Hit
GGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGCTT6509190.13648352292317173No Hit
CCCGGGATGGGGGCAGGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTG6487790.13603481157958583No Hit
CCCTGGCCAAAAGTGTTAGGTGAGCTACCATACTGGTGACAGTAAAACAC6439450.13502122717075676No Hit
GTTCCTAACACTCTGACTGGCCCTACAGGCGAGGGTGGCTCTTTCCCCTG6380880.1337931419654378No Hit
CTCCAGCTTGGTCCCCTGGCCAAAAGTGTTAGGTGAGCTACCATACTGGT6277230.13161982744381734No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6207120.13014977359807867No Hit
GCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCAGC6191810.1298287562770366No Hit
CCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGTTCCACGACACC6117330.12826707305879934No Hit
GTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCC6013520.12609040368601188No Hit
CACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGTCCTTGGGTT5990270.12560290187580758No Hit
CGTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGC5983810.12546744975994006No Hit
CCCCTGGCCAAAAGTGTTAGGTGAGCTACCATACTGGTGACAGTAAAACA5944680.12464697897141128No Hit
CTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACTCA5835380.12235519626795621No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA5817760.12198574328319062No Hit
GTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATG5798570.12158337082134889No Hit
CCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGG5729830.12014204288872765No Hit
CTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGACCT5691210.11933226568829366No Hit
CCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGC5688540.1192762816094444No Hit
CTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTTCTCGATGGGGGCTGGG5684190.11918507159334164No Hit
CCGGGATGGGGGCAGGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGG5677820.11905150658125557No Hit
CCGGGAGATCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTG5651970.11850948843958756No Hit
GGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCT5597740.11737240357217338No Hit
GCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCA5575630.11690880507653394No Hit
CCTGGCCAAAAGTGTTAGGTGAGCTACCATACTGGTGACAGTAAAACACT5327460.1117052212383231No Hit
CTCAGGGGTCCGGGAGATCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGA5313860.11142005888912834No Hit
CCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT5309720.1113332521151823No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA5125300.10746636678882197No Hit
CTGGGAAGTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCG5056760.10602923242015948No Hit
CTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCA5036960.10561406958824356No Hit
GGCTGTTCCTAACACTCTGACTGGCCCTACAGGCGAGGGTGGCTCTTTCC4919010.1031409152435727No Hit
CCTGGAAGTTCTGTGCGTAAGCTGTGCGACCATTATAAGTGCTGATCCAT4914380.1030438342379277No Hit
CTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGC4860180.10190737840510736No Hit
GTCTGGGATGCCAGTGGCCCTGATGGATGCAGCATAGATGAGGAGTCTGG4816770.1009971653478614No Hit
CCCCTCTCGCACAGAAATATATGGCCGCGTCGTCAAATCTCAGGCTCCTC4802080.10068914807509145No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)