FastQCFastQC Report
Fri 29 Jul 2022
EGAF00006158501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450084136
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTC21439180.476337161992308No Hit
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAA20520280.45592097918332314No Hit
CTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGC17603760.3911215391070793No Hit
GTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT15604990.3467127310614654No Hit
CCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACT15139730.33637555268111025No Hit
CCCTGCTGGACGCGTGATAGATGAGGAGCCGGGGAGGCTGGCCAGGCCTC14674710.3260437066371075No Hit
CGCGTGATAGATGAGGAGCCGGGGAGGCTGGCCAGGCCTCTGCTGGAACC13673960.30380897495129666No Hit
CTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTT13625140.3027242888649601No Hit
GGACGATCTCGCACAGTAATACATGGCGCTGTCTGAGGCCTTCAGGCTGC13571330.3015287346186314No Hit
GGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTC12936400.2874218166178601No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC12466370.27697865805250244No Hit
CTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACGTTGC11183830.2484830969470117No Hit
GGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATATCCACTTT11131150.2473126491176752No Hit
CCTCATCATAGTAATGGGACGATCTCGCACAGTAATACATGGCGCTGTCT11105080.2467334240814033No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11069400.24594068341035688No Hit
CTCAGATTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGA11009260.24460448879273541No Hit
CCCAGACCCACTGCCACTGAACCTGACTGGGATGCCAGTGGCCCTGCTGG10968090.2436897709276294No Hit
GTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCC10192170.22645032750054533No Hit
GTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATG10169290.2259419781016232No Hit
CTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCCCCCAGGAGTTCAG10007660.22235087174901005No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9966320.22143237681232114No Hit
CCGGGCTTTTTCACCTCTGCTCCAGACTGCATCAGCTGCACCTCGGCACA9908680.22015172736503646No Hit
CAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCG9724060.21604982762600639No Hit
GTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTG9226510.2049952278255815No Hit
CCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGG9212660.20468750758191576No Hit
CTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCTGGGATAGA9051430.2011052884565565No Hit
CTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTG8918690.19815606209235512No Hit
CTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCT8877340.19723734497498485No Hit
CGGGCTTTTTCACCTCTGCTCCAGACTGCATCAGCTGCACCTCGGCACAG8781520.19510840968631696No Hit
CTGGGAAGTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCG8677980.1928079509116491No Hit
GTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGT8663700.19249067689868546No Hit
CTGACACTCAGATTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGA8582620.19068923593432316No Hit
GTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGACTGCGTCAA8318210.18481455653882456No Hit
CAGAAGCCTTACAGGAGATCTTCAGAGACTCCCCGGGCTTTTTCACCTCT7884780.17518457926719727No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA7684110.17072607953460506No Hit
CGGGGAAGTAGTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAG7607470.1690232867927609No Hit
CAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACT7504870.16674371300213967No Hit
CGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATATCCACTT7435110.1651937805690623No Hit
CCAGGGCCGAAAGTCTCCGGTGAGTCACCATACTGCTGACAGTAATACAC7432220.165129570352153No Hit
CTCCGGTGAGTCACCATACTGCTGACAGTAATACACTGCAAAATCTTCAG7385240.16408576551118434No Hit
CACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGTCCTTGGGTT7314890.16252272441790752No Hit
CCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAG7281850.1617886394467367No Hit
CTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGACCT7168270.15926511126799636No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC6997760.15547670847034697No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA6985540.15520520367774082No Hit
CTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTTCTCGATGGGGGCTGGG6981140.1551074441779481No Hit
CGTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGC6949950.1544144626328265No Hit
CTGGGATGCCAGTGGCCCTGCTGGACGCGTGATAGATGAGGAGCCGGGGA6949050.15439446637150525No Hit
CTCACGTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAG6764470.15029345535520053No Hit
CCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGG6736220.14966579493039497No Hit
CCACAGTTCGTTTAATATCCACTTTGGTCCCAGGGCCGAAAGTCTCCGGT6659370.14795833639424252No Hit
CTGCTGGACGCGTGATAGATGAGGAGCCGGGGAGGCTGGCCAGGCCTCTG6650510.147761484310569No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG6617700.14703250949506916No Hit
CCGGGATGGGGGCAGGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGG6450230.14331164962454931No Hit
CCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGTTCCACGACACC6446820.14323588601220996No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT6438540.14305192040805456No Hit
CAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGG6434710.1429668252070986No Hit
CTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTT6287510.1396963255776693No Hit
CCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTATAGAGGAAGAAG6238880.13861586092427838No Hit
GGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATATCCACTTTG6189560.1375200658038745No Hit
GGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCA6112460.13580705275068838No Hit
CCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCAC6060660.1346561568213104No Hit
CTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGC6050430.13442886598429232No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGG5847860.12992815192224416No Hit
CGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5804140.12895677798339464No Hit
CACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAA5736150.12744617153091573No Hit
GCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCA5682280.1262492842004989No Hit
GTCATCTCCTCCCGGGATGGGGGCAGGGTGTACACCTGTGGTTCTCGGGG5635460.12520903425043178No Hit
CCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT5632710.12514793456306134No Hit
GTGATAGATGAGGAGCCGGGGAGGCTGGCCAGGCCTCTGCTGGAACCAGG5630170.12509150066999916No Hit
CTCAGGGGTCCGGGAGATCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGA5617260.12480466541038007No Hit
CTAAGTAGTTACTGCTGACACTCAGATTGGCCCTGCAGGAGAGGGTGGCT5612140.12469090890153037No Hit
GCCACAGTTCGTTTAATATCCACTTTGGTCCCAGGGCCGAAAGTCTCCGG5599090.12440096311237239No Hit
GGGACGATCTCGCACAGTAATACATGGCGCTGTCTGAGGCCTTCAGGCTG5505790.12232801735540397No Hit
CTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGG5481030.12177789798838855No Hit
GCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATATCCACT5457910.12126421625311405No Hit
CCGGGAGATCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTG5438890.12084162859719189No Hit
GCTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACGTTG5216560.11590188550880186No Hit
CCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGC5180120.11509225910597302No Hit
GTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTC5131410.11401001700713131No Hit
CTGGGCTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCT5128550.11394647333226604No Hit
CTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGT5082150.1129155549708155No Hit
CTCGCACAGTAATACATGGCGCTGTCTGAGGCCTTCAGGCTGCTCCACTG5050640.11221546364389079No Hit
CTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCA5046970.11213392333383641No Hit
GTTTAATATCCACTTTGGTCCCAGGGCCGAAAGTCTCCGGTGAGTCACCA4963800.11028604660707259No Hit
GGCCTTTCCCGGGCATCTGGCGCACCCAGGCGATCCAATAGTTGGTAAAT4959640.11019361944363221No Hit
CAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGA4934550.10963616811413233No Hit
CTCATCATAGTAATGGGACGATCTCGCACAGTAATACATGGCGCTGTCTG4927970.10948997322580593No Hit
GGGAGATCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGAC4869460.10818999405924407No Hit
CGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGTTCCACGACACCG4863870.10806579505837104No Hit
CCCGGGATGGGGGCAGGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTG4848520.10772474771250326No Hit
CGGGAGATCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGA4794310.10652030623892063No Hit
GTCAGAGTCATCGGGATTGACGATGCCCATCCACTCTAGGCCTTTCCCGG4788720.1063961072380476No Hit
GTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAG4765580.10588198114141043No Hit
CTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGG4723300.10494260122067489No Hit
CTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA4715280.10476441231423451No Hit
CTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAG4676880.10391123849786164No Hit
CTTTGGAGATGGTTTTCTCGATGGGGGCTGGGAGGGCTTTGTTGGAGACC4628270.10283121820583341No Hit
CTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCT4626710.10279655801954325No Hit
GCCGAAAGTCTCCGGTGAGTCACCATACTGCTGACAGTAATACACTGCAA4625000.10275856512303291No Hit
CTCTTGTCCACGGTGAGCTTGCTATAGAGGAAGAAGGAGCCGTCGGAGTC4601020.10222577584916255No Hit
CGGGGAGGCTGGCCAGGCCTCTGCTGGAACCAGGCTAAGTAGTTACTGCT4565460.10143570134629229No Hit
CGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGCTGACC4563500.10139215393274825No Hit
GTCCACGGTGAGCTTGCTATAGAGGAAGAAGGAGCCGTCGGAGTCCAGCA4516740.10035323706676921No Hit
TGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCT4509060.10018260230349466No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)