FastQCFastQC Report
Fri 22 Jul 2022
EGAF00006158502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450084136
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTAC25911750.5757090269895672No Hit
CTGGGATCTCAGGGCTTCATTTTCTGTCCTCCAGCATCATGGGGTCAACC20149250.4476774093633018No Hit
CTCAGGGCTTCATTTTCTGTCCTCCAGCATCATGGGGTCAACCGCCATCC18248840.4054539704994179No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC17169900.38148200806615407No Hit
CTTCACTCTCACTATCAGCAGAGTGGAGCCTGAAGATTTTGCAGTGTATT14780520.32839460042644114No Hit
TGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAT13949830.30993827340761904No Hit
CAGGGCTTCATTTTCTGTCCTCCAGCATCATGGGGTCAACCGCCATCCTC13171780.2926515054954081No Hit
GGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATA12670220.28150781124176305No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC11899030.26437345927695616No Hit
GAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTG11388830.2530378009146272No Hit
CGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCC11080530.24618797050869617No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATTAAACGAACTGTGGCTGCACCA10872740.24157127813098483No Hit
CCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAG10869340.24149573669932678No Hit
CCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTGACTCACCGGA10661950.23688793154886933No Hit
CTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTGACTCACCGGAG10383940.23071108642673865No Hit
CCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACT10358680.23014985802565588No Hit
GGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGC10263380.22803247613241806No Hit
ATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAG10101330.22443203819118834No Hit
CACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGT10056270.22343089204103828No Hit
CCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTG9851940.21889107417907305No Hit
CTGGCATCCCAGTCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT9805450.21785815619149038No Hit
CCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCA9725850.2160895979679675No Hit
CGGACACATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAG9712130.21578476607315927No Hit
CTCACACATGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCA9564010.21249382582104606No Hit
GTGGATATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC9114970.20251702450583597No Hit
CAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTC9089480.20195068594908222No Hit
TGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTGACTCACCGGAGA9044140.20094331873985447No Hit
CGGAGACTTTCGGCCCTGGGACCAAAGTGGATATTAAACGAACTGTGGCT8998390.19992684212269146No Hit
CTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGT8920470.19819561025363489No Hit
CCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGT8143610.1809352818425042No Hit
CCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACA8132390.18068599511803277No Hit
CGTCAATCCCGATGACTCTGACACCAGATACAATTCGTCCTTCGAAGGCC7971840.17711888427900513No Hit
TGGATATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCA7929680.17618217052644575No Hit
CTCTGACACCAGATACAATTCGTCCTTCGAAGGCCAGGTCACCATCTCAG7813190.1735939877694334No Hit
CTCACCGGAGACTTTCGGCCCTGGGACCAAAGTGGATATTAAACGAACTG7812660.17358221219332198No Hit
CCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAATC7810700.17353866477977797No Hit
CGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACC7753900.17227667850972644No Hit
CTGGGACAGACTTCACTCTCACTATCAGCAGAGTGGAGCCTGAAGATTTT7537420.16746691111992448No Hit
GTCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACTATCAG7501770.16667483699092206No Hit
GGGAACCCTGGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCT7261890.1613451668067679No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7235680.16076283124095714No Hit
CCTGGGTGCGCCAGATGCCCGGGAAAGGCCTAGAGTGGATGGGCATCGTC6864760.15252170540843057No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6736970.149682458481496No Hit
CAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACG6680620.1484304703421051No Hit
AGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGG6396590.14211987244980348No Hit
CCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAG6349120.1410651807554488No Hit
CTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTC6326830.14056994001672612No Hit
CTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGA6306990.14012913354493348No Hit
CTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAATCT6205680.137878221062206No Hit
CCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGC6167240.13702415852310776No Hit
CGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCA6164120.13695483815052747No Hit
GGGATCTCAGGGCTTCATTTTCTGTCCTCCAGCATCATGGGGTCAACCGC6108150.13571129287702777No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGG6101530.13556420926597598No Hit
CGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGC6086820.13523738148371442No Hit
CACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAG6083210.1351571742577481No Hit
CACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGA5969550.132631868633557No Hit
CCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGA5881020.13066490306159112No Hit
CTTAGCCTGGTTCCAGCAGAGGCCTGGCCAGCCTCCCCGGCTCCTCATCT5873230.13049182431082174No Hit
TGGTGACTCACCGGAGACTTTCGGCCCTGGGACCAAAGTGGATATTAAAC5737810.12748305352401934No Hit
GTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGC5701790.1266827587098071No Hit
CCAAAGTGGATATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC5676250.12611530924964662No Hit
CCTAGAGTGGATGGGCATCGTCAATCCCGATGACTCTGACACCAGATACA5629420.12507483711889814No Hit
CTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAG5458270.12127221475764255No Hit
AGCAGGGCCACTGGCATCCCAGTCAGGTTCAGTGGCAGTGGGTCTGGGAC5362240.11913861367466637No Hit
CACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTG5351350.11889665891267939No Hit
AGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGC5341960.11868803125289445No Hit
GACAGACTTCACTCTCACTATCAGCAGAGTGGAGCCTGAAGATTTTGCAG5279480.11729984635583779No Hit
CCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAA5233650.11628159229322405No Hit
CCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGT5230790.11621804861835876No Hit
CATCTATCACGCGTCCAGCAGGGCCACTGGCATCCCAGTCAGGTTCAGTG5204880.11564237847298843No Hit
CAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAA5112510.11359009551938529No Hit
CTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGG5079520.11285712145162122No Hit
CAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCC5062130.11247074924675862No Hit
AACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAG4986060.11078062080375124No Hit
CCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGT4921970.1093566648169977No Hit
GAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4893950.10873411454786312No Hit
GGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCCCCTCCCAT4869190.10818399518084769No Hit
CTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTG4835050.10742547033472871No Hit
CTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTAC4807740.10681869489396977No Hit
CATTTACCAACTATTGGATCGCCTGGGTGCGCCAGATGCCCGGGAAAGGC4761370.10578844307456328No Hit
GCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATA4722460.10492393804344173No Hit
CTTCTGGATACACATTTACCAACTATTGGATCGCCTGGGTGCGCCAGATG4718410.10483395486749615No Hit
CTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCA4712600.1047048678916335No Hit
CACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACA4666500.10368061495062338No Hit
GTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCC4655820.10344332598294466No Hit
ATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA4635870.1030000755236572No Hit
CAGATATCACCGGACACATTGTGTTGACGCAGTCTCCAGGCACCCTGTCT4590750.10199759628941911No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)