FastQCFastQC Report
Sat 28 May 2022
EGAF00006158531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158531
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences31858938
Sequences flagged as poor quality0
Sequence length41
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC3253211.0211294550998529No Hit
ATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTAC2759600.8661933426657222No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG2593360.8140133233568551No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACA2141510.6721849924815447No Hit
TGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCC1461070.45860599621996184No Hit
TGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATT1329540.41732087868089013No Hit
CTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT1223900.38416220904789733No Hit
TGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGC1059340.3325095142845No Hit
CTCAGCATTTGGGGACGCTCTCAGCTCTCGGCGCACGGCCC733880.23035293894605025No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTG655790.20584176409144586No Hit
CCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGG626830.19675169335525247No Hit
TGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAA615340.19314517012462876No Hit
ACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTAC595220.18682983092531208No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGT584060.18332688930183422No Hit
TTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGA580170.18210588187214527No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTA567720.17819803032982456No Hit
GCTCAGCATTTGGGGACGCTCTCAGCTCTCGGCGCACGGCC561810.17634297791093978No Hit
GTTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCTC523190.16422079103829512No Hit
TAAACTTCGCCTTAATTTTAATAATCAACACCCTCCTAGCC481130.15101884438206947No Hit
GCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAG468960.14719888026399375No Hit
GTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG428670.13455250768245947No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGC416280.1306634891596198No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCA405960.12742420980887686No Hit
AGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT393750.1235916903444804No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAG384840.12079498695154245No Hit
CCATTACTGCAGGAAAAGGTCCCGGAGAGCTGAGCAGTCAA373000.11707860444061255No Hit
CTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA354100.11114620330407748No Hit
TTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC350580.1100413328278551No Hit
ATCCCCTCGACCGCTCGCGTCGCATTTGGCCGCCTCCCTAC350170.10991264052806782No Hit
GCCATTACTGCAGGAAAAGGTCCCGGAGAGCTGAGCAGTCA348860.10950145293606461No Hit
TGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC344290.10806700461892357No Hit
TTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCTCG342560.10752398589055291No Hit
TCCCCTCGACCGCTCGCGTCGCATTTGGCCGCCTCCCTACC327290.1027309824326222No Hit
TCTCTCTCGGGTGGAGTCTTCTGACAGCTGGTGCGCCTGCC326060.1023449055332604No Hit
CCCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAG325330.10211577046290747No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)