FastQCFastQC Report
Sat 28 May 2022
EGAF00006158534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158534
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences31470338
Sequences flagged as poor quality0
Sequence length36
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACC3827121.2161038753381042No Hit
CTTAATCAACGCAAGCTTATGACCCGCACTTACTGG2653530.8431844615078491No Hit
TGACCCACCAATCACATGCCTATCATATAGTAAAAC1850510.5880171989255406No Hit
ATGCCCCAACTAAATACTACCGTATGGCCCACCATA1751960.5567019966547547No Hit
TGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTA1593330.5062958014623167No Hit
GGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAG1425020.45281369396159643No Hit
ACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCT1175670.3735803536650925No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGA1113560.35384430888540186No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGC1075790.3418425312114538No Hit
TGACCCCAATACGCAAAACTAACCCCCTAATAAAAT855170.27173842238364265No Hit
TAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTA798250.2536515495956859No Hit
CTCAGCATTTGGGGACGCTCTCAGCTCTCGGCGCAC783300.2489010445327915No Hit
TGATGTTCGCCGACCGTTGACTATTCTCTACAAACC686470.21813238866389045No Hit
GCTCAGCATTTGGGGACGCTCTCAGCTCTCGGCGCA683960.21733481222858173No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTG563490.1790543209291238No Hit
CATACCCATGGCCAACCTCCTACTCCTCATTGTACC494310.15707171623005764No Hit
AGGGACTTAATCAACGCAAGCTTATGACCCGCACTT423650.13461882741774175No Hit
TAAACTTCGCCTTAATTTTAATAATCAACACCCTCC397940.1264492297477072No Hit
TCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGC384210.1220863913187078No Hit
GACAACTCGGTGGTGGCCACTGCGCAGACCAGACTT368140.11697999557551624No Hit
AGAATCTTAGTTCAACTTTAAATTTGCCCACAGAAC361810.1149685777127656No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTG358270.11384370895539794No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTC354870.11276332653306742No Hit
TTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGT350910.11150499877058835No Hit
CCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTC343370.10910909186930245No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAG340970.1083464689829515No Hit
GTTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGG337270.10717075869982713No Hit
CCATTACTGCAGGAAAAGGTCCCGGAGAGCTGAGCA321050.10201669902623861No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)