FastQCFastQC Report
Sat 28 May 2022
EGAF00006158535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158535
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences32649103
Sequences flagged as poor quality0
Sequence length41
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT4264891.3062809106884192No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACA3220020.9862506789237058No Hit
GGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1919660.5879671487452504No Hit
ATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTAC1550470.47488900384185134No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1462020.44779790734220165No Hit
TGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCC1453160.445084203385312No Hit
TGGGCCTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCG1244170.38107325643831624No Hit
TGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGC872810.267330468466469No Hit
ATCCCCTCGACCGCTCGCGTCGCATTTGGCCGCCTCCCTAC675610.2069306467623322No Hit
TGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATT636630.19499157450053067No Hit
TCCCCTCGACCGCTCGCGTCGCATTTGGCCGCCTCCCTACC635500.19464547004553234No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTG607070.18593772698747651No Hit
AGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG585320.17927598194657904No Hit
TTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGA567960.1739588373989938No Hit
TGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAA547270.1676217567141125No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGT545480.16707350275442484No Hit
GTTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCTC537600.16465996018328588No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCA520360.15937957009109865No Hit
ATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCA512870.1570854795000034No Hit
GCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAG504520.1545279819785554No Hit
ACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTAC479570.14688611812704316No Hit
TCTCTCTCGGGTGGAGTCTTCTGACAGCTGGTGCGCCTGCC468000.14334237605241404No Hit
TAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT458470.14042345971955186No Hit
TTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC455340.13946478100791926No Hit
CCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTG452020.1384479077419064No Hit
TAAACTTCGCCTTAATTTTAATAATCAACACCCTCCTAGCC438680.13436203745015599No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTA421970.12924397953597683No Hit
GTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG394030.12068631717079639No Hit
TCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCAT392370.12017788053778997No Hit
CCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGG388580.11901705232146807No Hit
CATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTC378480.11592355232546511No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG376630.11535692113807842No Hit
ATCTCTCTCGGGTGGAGTCTTCTGACAGCTGGTGCGCCTGC371760.11386530282317404No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGC352550.10798152708820208No Hit
CCATTACTGCAGGAAAAGGTCCCGGAGAGCTGAGCAGTCAA328210.10052649838496329No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)