FastQCFastQC Report
Sat 28 May 2022
EGAF00006158537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158537
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences26992419
Sequences flagged as poor quality0
Sequence length41
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT3116661.1546427165345943No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACA2267010.8398691499268739No Hit
GGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1815080.6724406582455614No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1390750.5152372597654178No Hit
TGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCC1264420.4684352299065897No Hit
ATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTAC1255860.46526396911666196No Hit
TGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGC732180.2712539398562241No Hit
ATCCCCTCGACCGCTCGCGTCGCATTTGGCCGCCTCCCTAC649990.2408046496314391No Hit
TCCCCTCGACCGCTCGCGTCGCATTTGGCCGCCTCCCTACC573680.2125337488277727No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTG546340.20240497896835402No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGT542070.20082305331730363No Hit
GTTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCTC537700.19920408022711858No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCA536220.19865577812792548No Hit
TGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATT532980.1974554410999622No Hit
ACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTAC524270.19422860915133247No Hit
TGGGCCTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCG516830.19147227967971303No Hit
TTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGA502990.18634491410347476No Hit
GCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAG501670.1858558879068971No Hit
TGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAA470250.17421558253078392No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTA460670.17066643786168256No Hit
AGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG451170.16714693114388895No Hit
TTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC408760.15143511220687556No Hit
TCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCAT397610.14730432274335992No Hit
TAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT395610.1465633739606665No Hit
TCTCTCTCGGGTGGAGTCTTCTGACAGCTGGTGCGCCTGCC393710.14585947261710777No Hit
CCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGG392000.14522596140790495No Hit
GTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG379940.14075804024826377No Hit
TAAACTTCGCCTTAATTTTAATAATCAACACCCTCCTAGCC376110.13933912332940593No Hit
ATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCA372480.13799430128881743No Hit
CCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTG365230.13530836195155388No Hit
CCATTACTGCAGGAAAAGGTCCCGGAGAGCTGAGCAGTCAA356390.1320333683320491No Hit
TTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCTCG340230.1260465021678865No Hit
CTCAGCATTTGGGGACGCTCTCAGCTCTCGGCGCACGGCCC330380.12239732941312152No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGC325640.12064128079813817No Hit
GCCATTACTGCAGGAAAAGGTCCCGGAGAGCTGAGCAGTCA325130.12045233885855137No Hit
ATCTCTCTCGGGTGGAGTCTTCTGACAGCTGGTGCGCCTGC306390.11350964876471427No Hit
CATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTC298920.11074220506135445No Hit
ATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTC291160.10786732378450407No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG278680.10324380338049732No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)