FastQCFastQC Report
Sat 28 May 2022
EGAF00006158571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158571
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences50029491
Sequences flagged as poor quality0
Sequence length41
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC4634750.9264035886353512No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG3928410.7852188622106909No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACA3731560.7458720697358284No Hit
CTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT3164640.6325549064650687No Hit
TGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCC2719960.5436713317750924No Hit
ATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTAC1987150.39719572601688075No Hit
TGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGC1819220.3636295240341342No Hit
TGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATT1463060.2924395133262499No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTG1158700.23160339568515698No Hit
TTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGA977060.19529681003550486No Hit
TGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAA974210.1947271460347258No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGT908490.1815908940588662No Hit
TGGGCCTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCG889820.1778590951484995No Hit
GCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAG855100.17091918844427179No Hit
GTTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCTC843490.1685985571989929No Hit
TAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT804620.16082913975678867No Hit
ACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTAC795840.15907417487017808No Hit
AGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT793780.15866241773277284No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTA738870.14768689131776294No Hit
TAAACTTCGCCTTAATTTTAATAATCAACACCCTCCTAGCC719740.14386314663884947No Hit
CTCAGCATTTGGGGACGCTCTCAGCTCTCGGCGCACGGCCC715620.14303963236403902No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCA682380.13639555117600535No Hit
GGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA658440.13161037356946126No Hit
TCTCTCTCGGGTGGAGTCTTCTGACAGCTGGTGCGCCTGCC642130.12835029642816073No Hit
GTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG638280.12758075032184515No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGC631310.1261875720462557No Hit
TTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC629390.12580379840362557No Hit
GCTCAGCATTTGGGGACGCTCTCAGCTCTCGGCGCACGGCC615290.12298546071556075No Hit
ATCTCTCTCGGGTGGAGTCTTCTGACAGCTGGTGCGCCTGC580110.11595360824278624No Hit
CCATTACTGCAGGAAAAGGTCCCGGAGAGCTGAGCAGTCAA541710.10827813539018416No Hit
AGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG538730.10768248671568535No Hit
GCCATTACTGCAGGAAAAGGTCCCGGAGAGCTGAGCAGTCA523650.10466826456419474No Hit
CTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTC510200.10197985024472866No Hit
CCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTG509110.10176197874969385No Hit
CCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGG505910.10112235601197701No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)