FastQCFastQC Report
Sat 28 May 2022
EGAF00006158596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158596
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences30099956
Sequences flagged as poor quality0
Sequence length41
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT2660460.8838750461960807No Hit
GGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2555690.8490676863447906No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACA2188790.7271738204534253No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG2117620.7035292676175341No Hit
TGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCC1534310.5097382866606184No Hit
ATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTAC1198290.39810357197864343No Hit
TGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGC1098970.3651068460033629No Hit
TGGGCCTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCG818030.27177116139305985No Hit
TGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATT777010.25814323449509363No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTG674860.22420630780988518No Hit
TTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGA604220.2007378349656059No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGT536120.17811321717546696No Hit
CTCAGCATTTGGGGACGCTCTCAGCTCTCGGCGCACGGCCC522120.1734620475857174No Hit
ACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTAC505640.16798695652578363No Hit
AGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG502210.16684741997629499No Hit
TGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAA498450.16559824871504794No Hit
GCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAG481940.16011319086313616No Hit
TAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT477330.15858162716251145No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTA445860.14812646237755298No Hit
GTTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCTC432840.14380087465908586No Hit
TCTCTCTCGGGTGGAGTCTTCTGACAGCTGGTGCGCCTGCC428320.1422992113343953No Hit
AGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT425390.14132578798454057No Hit
TAAACTTCGCCTTAATTTTAATAATCAACACCCTCCTAGCC415470.13803010210380373No Hit
GCTCAGCATTTGGGGACGCTCTCAGCTCTCGGCGCACGGCC399430.1327011906595478No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGA396370.13168457787778826No Hit
CCATTACTGCAGGAAAAGGTCCCGGAGAGCTGAGCAGTCAA395120.13126929487870348No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCA386950.12855500519668533No Hit
GGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA377000.1252493525239705No Hit
TTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC374520.12442543105378626No Hit
GTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG369400.122724431889535No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCA356210.11834236568319235No Hit
ATCTCTCTCGGGTGGAGTCTTCTGACAGCTGGTGCGCCTGC349410.11608322616817113No Hit
CCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGG341680.11351511610183085No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGC340540.11313637800666551No Hit
GCCATTACTGCAGGAAAAGGTCCCGGAGAGCTGAGCAGTCA336730.1118705954254551No Hit
CTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTC328290.10906660461563464No Hit
ATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCA327660.10885730198409592No Hit
CATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTC307320.10209981702298833No Hit
TCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCAT305590.101525065352255No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG305250.10141210837650394No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)