FastQCFastQC Report
Sat 28 May 2022
EGAF00006158602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158602
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences22473073
Sequences flagged as poor quality0
Sequence length41
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2830291.2594138772209746No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG2287311.0178002803621917No Hit
ATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTAC1777450.7909243208527823No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACA934710.41592442653481343No Hit
TGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATT814540.36245154367629207No Hit
CTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT810110.36048029568541873No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGT672560.29927371303426104No Hit
ACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTAC666570.29660830096533747No Hit
GCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAG636320.283147747528787No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTA616120.27415921267198307No Hit
TGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCC601350.26758690277916153No Hit
CTCAGCATTTGGGGACGCTCTCAGCTCTCGGCGCACGGCCC567930.2527157723378552No Hit
TGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGC499660.22233719438369642No Hit
GCTCAGCATTTGGGGACGCTCTCAGCTCTCGGCGCACGGCC490240.21814551129700865No Hit
TTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC439150.19541163774086434No Hit
GTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG424230.1887725813020765No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGC423730.18855009281552193No Hit
GTTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCTC410880.1828321387110699No Hit
TGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAA400660.17828447404589484No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTG384950.17129388579835075No Hit
ATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTC353020.15708577104697696No Hit
TTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGA322220.14338048027521647No Hit
TGGGCCTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCG313670.13957592715513362No Hit
CCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGG307810.13696836209271424No Hit
GGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTC306740.1364922367314875No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCA287640.12799317654510353No Hit
CTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCC248950.1107770174555122No Hit
TCTCTCTCGGGTGGAGTCTTCTGACAGCTGGTGCGCCTGCC237340.10561083479771548No Hit
TTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCT232960.10366183565549758No Hit
CTCTACCGGCGGGATTTGATGGCGTGATGTCTCACAGAAAG229960.10232690473617025No Hit
ATCCCCTCGACCGCTCGCGTCGCATTTGGCCGCCTCCCTAC227070.10104092128388495No Hit
CTCTCTCCAGGCGTCCTCGTGGAAGTGACATCGTCTTTAAA225820.10048470006749856No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)