FastQCFastQC Report
Wed 1 Jun 2022
EGAF00006158609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158609
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences39391832
Sequences flagged as poor quality0
Sequence length41
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT4702811.1938540964532953No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACA2160640.5484994960376557No Hit
GGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2095210.5318894536308949No Hit
AGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1629090.4135603543394479No Hit
TGGGCCTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCG1263720.3208076232656557No Hit
TGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCC1071980.2721325578358478No Hit
AGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG1034530.26262551079117114No Hit
ATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTAC976180.24781279530233577No Hit
TGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGC835930.2122089675849552No Hit
GTTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCTC668650.16974331125295214No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCA657210.16683915589404422No Hit
ACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTAC653840.1659836485898904No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGA648370.16459503584397903No Hit
CTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCA636500.1615817207993779No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG622600.1580530704944111No Hit
TCTCTCTCGGGTGGAGTCTTCTGACAGCTGGTGCGCCTGCC603370.1531713478063168No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTG596500.15142733143256704No Hit
TTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGA561980.14266409340900926No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTA560820.14236961611737176No Hit
TAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT560530.14229599679446236No Hit
GGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA537670.13649276327132995No Hit
TTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC514830.13069460694288096No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGT512240.13003711023137993No Hit
ATCTCTCTCGGGTGGAGTCTTCTGACAGCTGGTGCGCCTGC506740.12864088169344345No Hit
TGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATT492070.12491675939316556No Hit
TCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCAT473220.12013150340405596No Hit
GTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG467130.11858549762295899No Hit
ATCCCCTCGACCGCTCGCGTCGCATTTGGCCGCCTCCCTAC463780.11773506751348858No Hit
TTTCTTGCTGCAGCAACGCGAGTGGGAGCACCAGGATCTCG459950.11676278473161644No Hit
CTCAGCATTTGGGGACGCTCTCAGCTCTCGGCGCACGGCCC455100.11553156502089063No Hit
CCATTACTGCAGGAAAAGGTCCCGGAGAGCTGAGCAGTCAA454060.11526755089735354No Hit
AGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGC440560.11184044448605487No Hit
CCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGG430510.10928915415764363No Hit
GCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAG429610.1090606803968904No Hit
ATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCA427520.10853011355247454No Hit
TGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAA426760.10833718015450514No Hit
GCCATTACTGCAGGAAAAGGTCCCGGAGAGCTGAGCAGTCA426450.10825848363691234No Hit
TCCCCTCGACCGCTCGCGTCGCATTTGGCCGCCTCCCTACC411570.10448105079245869No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)