FastQCFastQC Report
Wed 1 Jun 2022
EGAF00006158769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14513606
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGCAGAGTGTAGATCT8231985.67190538312808Illumina Single End PCR Primer 1 (97% over 37bp)
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC1833491.2632904600000856No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1027980.7082871065950116No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT693840.47806175804965356No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAGCAGAGTGTAGATCTC589030.40584676199698405Illumina Single End PCR Primer 1 (97% over 36bp)
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT543380.374393517365705No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG430490.29661133146373136No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA427130.2942962624174861No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT217030.1495355461626835No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG213270.14694487365855186No Hit
GGCAGCAGTCTCTAGAACTTCTCCAGAAGGAAGCCCGCTGATGTCCTCTA200410.13808422248750588No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA177910.12258152798139899No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG151210.10418499716748546No Hit
TAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCG149480.10299301221212702No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)