FastQCFastQC Report
Wed 1 Jun 2022
EGAF00006158773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006158773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19213125
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAGAACGCGTGTAGATCT13381396.964712924107869Illumina Single End PCR Primer 1 (96% over 32bp)
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC2581061.3433837545948406No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1401820.7296158225171595No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT1295780.674424384372662No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAGAACGCGTGTAGATCTC1049380.5461787189746593Illumina Single End PCR Primer 1 (96% over 31bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA923180.48049445366123417No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT844960.439782700627826No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG527960.2747913210370515No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG366620.1908174750333431No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT329000.17123711004846948No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA311250.1619986337464624No Hit
GAAGAGCGTCGTGTAGGGAAAGAGTGTTAGAACGCGTGTAGATCTCGGTG291020.151469373149865Illumina Single End PCR Primer 1 (96% over 27bp)
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG251910.1311134966331609No Hit
AGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGA231250.12036043069516282No Hit
TAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCG228800.11908526072671675No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA209960.10927946390813571No Hit
GTGAGGGTGATGGAATTCCGAGAGTGGGGCACCCGATCTTCTCGAGGTCT195370.10168569662665497No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)